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| Caption | Schematic representation of generation of Apptm3.1Zhe (APP/hAbeta/mutC) allele. E15, E16, and E17 stand for exon 15, 16, and 17, respectively. E16* is modified exon 16 with Swedish mutation K595N/M596L (APP695 numbering), and three other mutations to humanize amyloid beta region: G601R, F606Y, R609H (corresponding to G5R, F10Y, and R13H in C99 numbering). E17* is modified exon 17 with Arctic mutation E618G, London mutation V624I, and silent mutation on G621S622 (GGTTCG to GGATCC) to create a BamHI site (corresponding to E22G, V46I, G25 and S25 in C99 numbering. E17* has additional frameshift mutation at I656. "truncE16" is modified Exon 16 that was truncated at BACE cleavage site, at the sequence coding K670M671, followed by stop codon. Restriction sites marked are: A: Asc I; B: BamHI; H: HindIII. Arrow stands for LoxP; and oval for FRT. NEO: Neo-resistant cassette; DT: Diphtheria toxin cassette. 5' and 3' southern blot probes are indicated below the wild-type allele. | ||||
| Copyright | This image is from Li H, J Biol Chem 2010 Oct 1;285(40):30598-605 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:166379 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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