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Tg(H2-K-HLA-G,B2M)1Alm
Transgene Detail
Nomenclature
Symbol: Tg(H2-K-HLA-G,B2M)1Alm
Name: transgene insertion 1, Andrew L Mellor
MGI ID: MGI:3806366
Synonyms: HLA-Gtg
Transgene: Tg(H2-K-HLA-G,B2M)1Alm  Location: unknown  
Transgene
origin
Strain of Origin:  CBA/Ca
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(H2-K-HLA-G,B2M)1Alm expresses 2 genes
 
Mutation detailsTwo transgenic constructs were co-injected into male pronuclei of CBA/Ca zygotes. The constructs encoded (i) a H-2Kb/HLA-G transgene with the human HLA-G heavy chain (HLA-G; major histocompatibility complex, class I, G) gene inserted between the mouse H-2Kb MHC class I promoter and coding sequence, and (ii) a human B2M transgene (designed with the human beta2-microglobulin gene). (A third transgene was also co-injected as well, but was found not to have integrated into the genome.) The mean copy number of HLA-G genomic DNA per haploid mouse genome was 3 copies. HLA-G expression is observed in pre-implantation embryos and a variety of tissues; expression in the reproductive organs (uterus, ovary, and testes), lung, and liver is much more similar to mouse expression of Qa-2 than to Kb (despite the presence of the H-2Kb promoter and downstream coding sequence), while HLA-G expression in spleen, thymus and kidney correlates better with Kb expression patterns. (J:140011, J:140016)
Phenotypes
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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:140011 Warner CM, et al., Symposium: innovative techniques in human embryo viability assessment. Soluble human leukocyte antigen-G and pregnancy success. Reprod Biomed Online. 2008 Oct;17(4):470-85
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory