Psen1tm1Mpm
Targeted Allele Detail
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Symbol: |
Psen1tm1Mpm |
Name: |
presenilin 1; targeted mutation 1, Mark P Mattson |
MGI ID: |
MGI:1930937 |
Synonyms: |
PS1KI, PS1M146V, PS-1 M146V KI, PS1M146VKI- |
Gene: |
Psen1 Location: Chr12:83734926-83781869 bp, + strand Genetic Position: Chr12, 38.84 cM
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Alliance: |
Psen1tm1Mpm page
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Amyloid beta deposits in Apptm3.1Zhe/Apptm3.1Zhe Psen1tm1Mpm/Psen1tm1Mpm mice
Show the 1 phenotype image(s) involving this allele.
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Allele Type: |
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Targeted |
Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: Point mutations were introduced into the coding region of exon 5 that altered the codons corresponding to amino acids 145 and 146 from isoleucine and methionine to valine and valine, respectively, and a BstEII restriction site. A lox-P flanked neomycin cassette was also introduced into exon 4. F2 mice exhibited the expected polymorphism of the targeted allele when genomic DNA was amplified with exon 5 specific primers and the products were digested with the appropriate restriction enzyme. Northern blot analysis of total brain RNA using a Psen1 specific antibody showed that the targeted allele was expressed at normal physiological levels in homozygous mutant mice.
(J:51950)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:51950 Guo Q, et al., Increased vulnerability of hippocampal neurons to excitotoxic necrosis in presenilin-1 mutant knock-in mice. Nat Med. 1999 Jan;5(1):101-6 |
All: |
551 reference(s) |
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