Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:98919
Cobb J, et al., Comparative analysis of genes downstream of the Hoxd cluster in developing digits and external genitalia. Development. 2005 Jul;132(13):3055-67
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3689897
|
Gene symbol:
|
Epha3
|
Gene name:
|
Eph receptor A3
|
Probe:
|
Epha3 cDNA1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Results
|
Specimen
5B Wild type left:
embryonic day 12.5
(more )
Specimen
5B Wild type left:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5B HoxD Del1-13 left:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Hoxdtm21(Hoxd11)Ddu/Hoxdtm21(Hoxd11)Ddu
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5B Wild type right:
embryonic day 13.5
(more )
Specimen
5B Wild type right:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5B HoxD Del1-13 right:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Hoxdtm21(Hoxd11)Ddu/Hoxdtm21(Hoxd11)Ddu
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6B Wild type:
embryonic day 11.5
(more )
Specimen
6B Wild type:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: handplate |
Present |
Regionally restricted |
6B Wild type
|
Expression was detected in the most anterior part of the handplate.
|
Specimen
6B HoxD Del1-10/Del1-10:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS19: handplate |
Absent |
|
6B HoxD Del1-10/Del1-10
|
Expression was lost in the most anterior part of the handplate where the mutant Hoxd13 expression is gained.
|
Specimen
7A:
embryonic day 12.5
(more )
Specimen
7A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
7B:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Hoxdtm21(Hoxd11)Ddu/Hoxd+ Hoxa13tm2Ipc/Hoxa13+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
|
7B
|
Expression is moderately gained compared to wild type.
|
Specimen
7C:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
|
7C
|
The expression domain is much larger distally and posteriorly compared to the Hoxd mutant only (compare with Fig. 5B).
|
Specimen
7D:
(close )
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb |
Present |
|
7D
|
The expression domain was further gained to the posterior of the forelimb bud compared to Fig. 7C.
|
|