Probe:	Punc cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	1B	Expression in ventral interneurons and cells of the ventral-medial zone, no expression in motor neurons.
TS17: dorsal root ganglion	Present		1B

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present		2A

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		2B

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		2C

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2D
TS15: trunk mesenchyme	Present	Regionally restricted	2D	Expression in lateral plate mesoderm.
TS15: future forebrain	Absent		2D	Absent in ventral prosencephalon.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		2E
TS15: trunk mesenchyme	Present	Regionally restricted	2E	Expression in lateral plate mesoderm.
TS15: future forebrain	Absent		2E	Absent in ventral prosencephalon.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		2F	Staining decreased in the dorsal mesencephalon.
TS17: diencephalon roof plate	Present		2F
TS17: diencephalon floor plate	Absent		2F
TS17: head mesenchyme	Present		2F	Expression in the cranial mesenchyme.
TS17: 1st branchial arch	Present		2F

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Present		2G
TS17: rhombomere 3	Absent		2G

Structure	Level	Pattern	Image	Note
TS19: tail neural tube	Present	Regionally restricted	2H	Absent in motor neurons.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present		2I
TS17: hindbrain	Present		2I
TS17: midbrain	Present		2I
TS17: telencephalon	Present		2I

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2J	There is a regional restriction that is not detailed.
TS17: telencephalon	Present	Regionally restricted	2J	Restricted in the caudal-medial telencephalon.
TS17: hindbrain	Present	Regionally restricted	2J
TS17: midbrain	Present	Regionally restricted	2J

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong		2K

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Strong		2L
TS17: trunk mesenchyme	Strong	Regionally restricted	2L	Expression in lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Absent		2M
TS19: diencephalon lateral wall ventricular layer	Present		2M
TS19: metencephalon alar plate ventricular layer	Present		2M
TS19: metencephalon basal plate ventricular layer	Present		2M
TS19: midbrain ventricular layer	Present		2M

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Absent		2N
TS19: diencephalon lateral wall ventricular layer	Present		2N
TS19: metencephalon alar plate ventricular layer	Present		2N
TS19: metencephalon basal plate ventricular layer	Present		2N

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	2O	Expression is absent at the cervical level (shown) but is present at the lumbar levels (shown in 2P).
TS19: forelimb bud	Absent		2O

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Absent		2P
TS19: dorsal root ganglion	Present	Regionally restricted	2P	Expression is present at lumbar levels, absent in motor neurons.
TS19: neural tube	Present	Regionally restricted	2P	Expression is present at lumbar levels, absent in motor neurons.