Probe:	Pnk3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: embryo	Weak	Regionally restricted	2A	Expression is in scattered cells in the posterior half of the embryo.

Structure	Level	Pattern	Image	Note
TS11: node	Present		2B
TS11: allantois	Present	Regionally restricted	2B	Expression is in the base of the allantois and the extraembryonic mesoderm at the posterior end of the embryo.
TS11: extraembryonic mesoderm	Present	Regionally restricted	2B	Expression is in the base of the allantois and the extraembryonic mesoderm at the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS11: node	Present		2C
TS11: allantois	Present	Regionally restricted	2C	Expression is in the base of the allantois and the extraembryonic mesoderm at the posterior end of the embryo.
TS11: extraembryonic mesoderm	Present	Regionally restricted	2C	Expression is in the base of the allantois and the extraembryonic mesoderm at the posterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS11: heart	Present		2D
TS11: embryo	Present	Regionally restricted	2D	Expression is in the posterior trunk of the embryo. Expression in the node has gradually disappeared.

Structure	Level	Pattern	Image	Note
TS15: heart	Present	Regionally restricted		Expression was detected in the myocardial layer.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	2E	Expression in the poserior trunk and tail bud.
TS12: heart	Strong		2E
TS12: head somite	Weak	Regionally restricted	2E	Expression in most rostral somites at the dorsal-most aspect, probably marking the future dermatome.

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present		2F
TS14: head somite	Present	Graded	2F	Expression is weak in newly forming and stronger in more mature somites.
TS14: embryo	Present	Regionally restricted	2F	Expression is in the posterior trunk of the embryo.
TS14: nephric duct	Weak		2F

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	2G	Expression was detected in the distal domain.
TS15: nephric duct	Present		2G	Expression accompanies the posterior elongation of the duct.
TS15: trunk somite	Present	Graded	2G	Expression is weak in newly forming and stronger in more mature somites.
TS15: tail	Present		2G
TS15: embryo	Present	Regionally restricted	2G	Expression is in the posterior trunk of the embryo.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		2H
TS17: 2nd branchial arch	Present		2H
TS17: forelimb bud apical ectodermal ridge	Present		2H
TS17: hindlimb bud apical ectodermal ridge	Present		2H
TS17: tail	Present		2H
TS17: forelimb bud	Present	Regionally restricted	2H	Expression is in the condensing skeletal rudiments (and later in the pericondrium).
TS17: hindlimb bud	Present	Regionally restricted	2H	Expression is in the condensing skeletal rudiments (and later in the pericondrium).
TS17: trunk somite	Present	Graded	2H	Expression is very strong in newly forming somites at the tail tip, and in rostral somites it is resricted to the caudal half.
TS17: embryo	Present	Regionally restricted	2H	Expression is in the posterior trunk of the embryo.

Structure	Level	Pattern	Image	Note
TS17: mesonephros	Strong	Regionally restricted	3A	Expression is in the epithelium of the ureter adjacent to the metanephritic mesenchyme.

Structure	Level	Pattern	Image	Note
TS18: ureteric bud	Present	Regionally restricted	3B	Expression is only detected at the rounded tip of the bud and is absent from the stalk.

Structure	Level	Pattern	Image	Note
TS18: ureteric bud	Present	Regionally restricted	3C	Expression is only detected at the rounded tip of the bud and is absent from the stalk.

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Present	Regionally restricted	3D	The expression domain splits in two after bifurcation of the ureter. Expression remains at the rounded tip of both branches and is absent from the stalk.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present	Spotted	3E

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Spotted

Structure	Level	Pattern	Image	Note
TS26: metanephros	Present	Spotted

Structure	Level	Pattern	Image	Note
TS24: ureter	Present	Regionally restricted	3F	Expression was detected in the growing tips in the peripheral nephrogenic region.

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	4A	Expression was absent from the caudal somites.
TS19: ureteric bud	Present	Regionally restricted	4A	Expression in the tips was as in wild type in 12/26 cases, bilateral but with unequal staining in 5/26 cases and unilateral in 5/26 cases. Expression in the ureter tips was weak or not detected in 4/26 cases.

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	4B	Expression was absent from the caudal somites.
TS19: ureteric bud	Present	Regionally restricted	4B	Expression in the tips was as in wild type in 12/26 cases, bilateral but with unequal staining in 5/26 cases and unilateral in 5/26 cases. Expression in the ureter tips was weak or not detected in 4/26 cases.

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	4C	Expression was partially lost in some mutants and completely lost in others.
TS19: tail	Present		4C

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Weak	Regionally restricted	4D	Expression was detected in the tips bilaterally in 2/13 mutants.

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	4E	Expression was partially lost in some mutants and completely lost in others.
TS19: tail	Present		4E

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Weak	Regionally restricted	4F	Expression was detected in the tips of an unbranched ureter unilaterally in 3/13 mutants.

Structure	Level	Pattern	Image	Note
TS19: trunk somite	Present	Regionally restricted	4G	Expression was partially lost in some mutants and completely lost in others.
TS19: tail	Present		4G

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Absent		4H	The ureter was absent in 8/13 mutants.

Structure	Level	Pattern	Image	Note
TS20: ureter	Present	Regionally restricted	5A	Expression is in the ureter tip.

Structure	Level	Pattern	Image	Note
TS20: ureter	Present	Regionally restricted	5B	Expression is in the ureter tips as wild type, but the number of branches is decreased in the mutant.

Structure	Level	Pattern	Image	Note
TS22: ureter	Present	Regionally restricted	5C	Expression is in the ureter tip.

Structure	Level	Pattern	Image	Note
TS22: ureter	Present	Regionally restricted	5D	Expression is in the ureter tip as in wild type but mutants lack the half-moon form indicative of tip branching.