Sox17 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:312380 Sun-Wada GH, et al., Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep. 2021 Sep 29;11(1):19355
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7541133
Gene symbol:	Sox17
Gene name:	SRY (sex determining region Y)-box 17

Antibody:	anti-Sox17
Detection system:	Secondary antibody coupled to Cy3

Results

S3a WT left ©

S3a WT right ©

Specimen S3a WT: embryonic day 3.5 (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S3a WT left , S3a WT right

S3a -/- left ©

Specimen S3a -/-: embryonic day 3.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		S3a -/- right , S3a -/- left	Similar as in the wild-type.

S3b WT left ©

S3b WT right ©

Specimen S3b WT: embryonic day 4.5 (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	S3b WT left , S3b WT right	Expressed in cells on the surface of the inner cell mass.

S3b -/- left ©

Specimen S3b -/-: embryonic day 4.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		S3b -/- right , S3b -/- left	Similar as in the wild-type.

S3c WT left ©

S3c WT right ©

Specimen S3c WT: embryonic day 5.0 (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3c WT right , S3c WT left	visceral endoderm

S3c -/- left ©

Specimen S3c -/-: embryonic day 5.0; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Present		S3c -/- right , S3c -/- left	Positive cells did not exhibit epithelial structure but they appeared as a clump, and exhibited defects in apical-basolateral polarity.

S3d WT left ©

S3d WT right ©

Specimen S3d WT: embryonic day 5.5 (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: extraembryonic visceral endoderm	Present		S3d WT left , S3d WT right
TS7: embryonic visceral endoderm	Ambiguous		S3d WT left , S3d WT right	Downregulated expression, trace or absent.

S3d -/- left ©

Specimen S3d -/-: embryonic day 5.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: red - Sox17; blue - Gata6 (assay MGI:7541116); green - Prkcz (assay MGI:7541115).

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Strong		S3d -/- right , S3d -/- left	Positive cells did not exhibit epithelial structure but they appeared as a clump, and exhibited defects in apical-basolateral polarity.

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