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Gene Expression Data
Assay Details
Assay
Reference: J:312380 Sun-Wada GH, et al., Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep. 2021 Sep 29;11(1):19355
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7541099
Gene symbol: Gata6
Gene name: GATA binding protein 6
Antibody: anti-GATA6
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1b/d: embryonic day 5.2 (more )
Note: Triple labeled: green - Gata6; red - Cdx2 (assay MGI:7541055); blue - Pou5f1 (assay MGI:7541102).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1d , 1b Positive visceral endoderm layer seemed to be tightly sealed, and simple epithelium surrounded the epiblast and extraembryonic ectoderm.
Specimen 1g/i: embryonic day 5.2; Atp6v0ctm1.1Ywd/Atp6v0ctm1.1Ywd (more )
Note: Triple labeled: green - Gata6; red - Cdx2 (assay MGI:7541055); blue - Pou5f1 (assay MGI:7541102).
Structure Level Pattern Image Note
TS7: primitive endoderm Present 1i , 1g A few regions were positive for both OCT3/4 and GATA6, indicating that the patterning of epiblast and visceral endoderm was impaired. Positive cells did not exhibit epithelial structure but they appeared as a clump.
Specimen S2b/d: embryonic day 4.5 (more )
Note: Triple labeled: green - Gata6; red - Cdx2 (assay MGI:7541055); blue - Pou5f1 (assay MGI:7541102).
Structure Level Pattern Image Note
TS6: primitive endoderm Present S2b , S2d
Specimen S2g/i: embryonic day 4.5; Atp6v0ctm1.1Ywd/Atp6v0ctm1.1Ywd (more )
Note: Triple labeled: green - Gata6; red - Cdx2 (assay MGI:7541055); blue - Pou5f1 (assay MGI:7541102).
Structure Level Pattern Image Note
TS6: primitive endoderm Present S2g , S2i Similar as in the wild-type.
Specimen S6a/b: embryonic day 4.5 (more )
Note: Triple labeled: green - Gata6; red - Atp6v0c (assay MGI:7541123); blue - Pou5f1 (assay MGI:7541102).
Structure Level Pattern Image Note
TS6: parietal endoderm Present S6b , S6a
TS6: primitive endoderm Present S6b , S6a
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory