Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:312380 Sun-Wada GH, et al., Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep. 2021 Sep 29;11(1):19355
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7541102
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	Anti-Oct3/4
Detection system:	Secondary antibody coupled to Cy5

Results

1c ©

1d ©

Specimen 1c/d: embryonic day 5.2 (more

)
Note: Triple labeled: blue - Pou5f1; red - Cdx2 (assay MGI:7541055); green - Gata6 (assay MGI:7541099).

Structure	Level	Pattern	Image	Note
TS7: epiblast	Present		1d , 1c

1h ©

1i ©

Specimen 1h/i: embryonic day 5.2; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Pou5f1; red - Cdx2 (assay MGI:7541055); green - Gata6 (assay MGI:7541099).

Structure	Level	Pattern	Image	Note
TS7: epiblast	Present		1h , 1i	Reduced in size compared with the wild-type.

S2c ©

S2d ©

Specimen S2c/d: embryonic day 4.5 (more

)
Note: Triple labeled: blue - Pou5f1; red - Cdx2 (assay MGI:7541055); green - Gata6 (assay MGI:7541099).

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		S2c , S2d

S2h ©

S2i ©

Specimen S2h/i: embryonic day 4.5; Atp6v0c^tm1.1Ywd/Atp6v0c^tm1.1Ywd (more

)
Note: Triple labeled: blue - Pou5f1; red - Cdx2 (assay MGI:7541055); green - Gata6 (assay MGI:7541099).

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		S2h , S2i	Similar as in the wild-type.

S6a ©

S6b ©

Specimen S6a/b: embryonic day 4.5 (more

)
Note: Triple labeled: blue - Pou5f1; red - Atp6v0c (assay MGI:7541123); green - Gata6 (assay MGI:7541099).

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		S6a , S6b

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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