Sox9 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:238708 Gonen N, et al., Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone. PLoS Genet. 2017 Jan;13(1):e1006520
Assay type:	RT-PCR
MGI Accession ID:	MGI:7537171
Gene symbol:	Sox9
Gene name:	SRY (sex determining region Y)-box 9

Probe:	Sox9-pG, Sox9-pH
Probe preparation:	labelled with SYBR green
Assay notes:	Two primer sets were listed: MGI:3706532 and MGI:3510977. The set used in this assay was not specified. Quantitative RT-PCR. Relative expression was normalized to Hprt. Note: the last column was light grey (indicating XX) but was labeled as XY.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
XY TES+/+ Sox8+/+	E14.5	TS22: testis	Present	0.1-0.2 µg; total RNA	Not Specified		Male
XY TES +/+ Sox8-/-	E14.5	TS22: testis	Present (a)	0.1-0.2 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J * CBA	Sox8^tm1Weg/Sox8^tm1Weg	Male
XY TES -/+ Sox8-/-	E14.5	TS22: testis	Present (b)	0.1-0.2 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J * CBA	Rr505^em3Rlb/Rr505⁺, Sox8^tm1Weg/Sox8^tm1Weg	Male
XY TES -/- Sox8-/-	E14.5	TS22: testis	Present (c)	0.1-0.2 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J * CBA	Sox8^tm1Weg/Sox8^tm1Weg, Rr505^em3Rlb/Rr505^em3Rlb	Male
XY TES +/+ Sox8-/-	E14.5	TS22: gonad	Present	0.1-0.2 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6J * CBA	Sox8^tm1Weg/Sox8^tm1Weg	Not Specified

Notes:
(a) Sox9 mRNA levels were increased by about 2-fold upon deletion of Sox8 (TES+/+;Sox8+/+ vs. TES+/+;Sox8-/-).
(b) Expression levels were lowered to 77% in TES+/-;Sox8-/- compared to TES+/+;Sox8-/- levels.
(c) Expression levels were lowered to 50% in TES-/-;Sox8-/- compared to TES+/+;Sox8-/- levels.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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