Probe:	IMAGE clone 1094838
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Strong	Regionally restricted	1A	Expression was detected in the developing dorsal telencephalon, except in the dorsal midline.
TS15: optic stalk	Present		1A
TS15: 1st branchial arch mandibular component	Present		1A
TS15: 1st branchial arch maxillary component	Present		1A

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Strong	Regionally restricted	1B	Expression was detected in the developing dorsal telencephalon, except in the dorsal midline.
TS15: embryo ectoderm	Present	Regionally restricted	1B	Expression was detected in the lateral head ectoderm.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		1C
TS17: midbrain	Present	Regionally restricted	1C	Expression was detected in the ventral midbrain.
TS17: eye	Present		1C

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		1D
TS17: midbrain	Present	Regionally restricted	1D	Expression was detected in the ventral midbrain.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Graded	1E	Expression was detected in a high-caudomedial to low-rostrolateral gradient in the telencephalic evagination and was restricted to progenitors. Staining included the primordia of the choroid plexus. No expression was detected in the roofplate.
TS17: cortical hem	Present		1E
TS17: future pituitary	Present		1E

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	1F	Expression was detected in a high-caudomedial to low-rostrolateral gradient in the telencephalic evagination and was restricted to progenitors. Staining included the primordia of the choroid plexus. No expression was detected in the roofplate.
TS17: olfactory placode	Present		1F

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1G	Expression was detected in scattered cells located in the most marginal portion of the cerebral walls, most abundant in the medial area.
TS21: choroid plexus	Weak		1G

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1H	Expression was detected in scattered cells located in the most marginal portion of the cerebral walls, most abundant in the medial area.
TS21: choroid plexus	Weak		1H

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Graded	8M left

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Graded	8M right

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Graded	8N left	Graded expression was similar to that in normal embryos, but a downregulation in the rostral telencephalon was noted.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Graded	8N right	Graded expression was similar to that in normal embryos.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Graded	8O left	Graded expression was similar to that in normal embryos.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Graded	8O right	Graded expression was similar to that in normal embryos.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	8P left	The graded expression appeared to spread slightly more anteriorly and laterally, thus flattening its gradient in the Pax6<sey/sey> mutant.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	8P right	The graded expression appeared to spread slightly more anteriorly and laterally, thus flattening its gradient in the Pax6<sey/sey> mutant.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	S1A	Expression was detected in the developing dorsal telencephalon, except in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	S1B	Expression was detected in the developing dorsal telencephalon, except in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS20: cortical hem	Present		S1C

Structure	Level	Pattern	Image	Note
TS20: cortical hem	Present		S1D

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	S1F , S1E	Expression was detected in apical but not basal telencephalic progenitors.

Structure	Level	Pattern	Image	Note
TS22: telencephalon marginal layer	Present	Scattered	S1G	Expression was detected in scattered cells in the outer border of the marginal layer.
TS22: thalamus	Present		S1G

Structure	Level	Pattern	Image	Note
TS22: telencephalon marginal layer	Present	Scattered	S1H	Expression was detected in scattered cells in the outer border of the marginal layer.
TS22: thalamus	Present		S1H
TS22: telencephalon	Present	Regionally restricted	S1H	Expression was also detected in the developing dentate gyrus.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: telencephalon marginal layer	Cajal-Retzius cell	Present	Regionally restricted	S1I , S1J	Expression was detected in the outer border of the marginal layer; co-expression was detected with Reelin immunopositive cells.