Gene Expression Data
Assay Details
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Assay
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Reference:
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J:215514
Saulnier A, et al., The doublesex homolog Dmrt5 is required for the development of the caudomedial cerebral cortex in mammals. Cereb Cortex. 2013 Nov;23(11):2552-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7518826
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Gene symbol:
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Eomes
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Gene name:
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eomesodermin
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Results
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Specimen
3E Dmrt5+/+:
embryonic day 11.5
(more  )
Specimen
3E Dmrt5+/+:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3E Dmrt5+/+
,
3E' Dmrt5+/+
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Expression was detected in basal progenitors.
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Specimen
3E Dmrt5-/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
3E Dmrt5-/-
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3E' Dmrt5-/-
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At E11.5, there was a 3.15-fold increase in immunopositive cells in the mutants compared to controls.
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Specimen
3F Dmrt5+/+:
embryonic day 14.5
(more )
Specimen
3F Dmrt5+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
3F' Dmrt5+/+
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3F Dmrt5+/+
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Expression was detected in basal progenitors.
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Specimen
3F Dmrt5-/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
3F' Dmrt5-/-
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3F Dmrt5-/-
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At E14.5, a small increase of 1.25-fold in immunopositive cells was detected in the mutant compared with control.
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Specimen
3G Dmrt5+/+:
embryonic day 18.5
(more )
Specimen
3G Dmrt5+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
3G' Dmrt5+/+
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3G Dmrt5+/+
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Expression was detected in basal progenitors.
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Specimen
3G Dmrt5-/-:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
3G Dmrt5-/-
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3G' Dmrt5-/-
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No difference in the number of immunopositive cells was detected between the mutant and control.
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Specimen
S1E:
embryonic day 14.5
(more )
Note: Double labeled: brown - Eomes; blue - Dmrta2 (assay MGI:7518810).
Specimen
S1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Eomes; blue - Dmrta2 (assay MGI:7518810).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
S1E
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S1F
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Expression was detected in basally located intermediate progenitors.
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Analysis Tools