Tubb3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199486 Watanabe Y, et al., Sox10 and Itgb1 interaction in enteric neural crest cell migration. Dev Biol. 2013 Jul 1;379(1):92-106
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7513009
Gene symbol:	Tubb3
Gene name:	tubulin, beta 3 class III

Antibody:	anti-Tuj1
Detection system:	Secondary antibody

Results

1A Wild-type s ©

Specimen 1A WT s: embryonic day 13.5 (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A Wild-type s	Fully colonized enteric neural crest cells.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A Wild-type s	Fully colonized enteric neural crest cells.
TS21: stomach	migratory enteric neural crest cell	Present	1A Wild-type s	Fully colonized enteric neural crest cells.

1A Wild-type si ©

Specimen 1A WT si: embryonic day 13.5 (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A Wild-type si	Fully colonized enteric neural crest cells.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A Wild-type si	Fully colonized enteric neural crest cells.
TS21: small intestine	migratory enteric neural crest cell	Present	1A Wild-type si	Fully colonized enteric neural crest cells.

1A Wild-type ce ©

Specimen 1A WT ce: embryonic day 13.5 (more

)

Structure	Cell Type	Level	Image	Note
TS21: cecum	migratory enteric neural crest cell	Present	1A Wild-type ce	Fully colonized enteric neural crest cells.
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A Wild-type ce	Fully colonized enteric neural crest cells.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A Wild-type ce	Fully colonized enteric neural crest cells.

1A Wild-type co ©

Specimen 1A WT co: embryonic day 13.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	1A Wild-type co	Fully colonized enteric neural crest cells in the colon.
TS21: large intestine	migratory enteric neural crest cell	Present	Regionally restricted	1A Wild-type co	Fully colonized enteric neural crest cells in the colon.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	1A Wild-type co	Fully colonized enteric neural crest cells in the colon.

1A beta1 neo/+ s ©

Specimen 1A beta1 neo/+ s: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A beta1 neo/+ s	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A beta1 neo/+ s	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: stomach	migratory enteric neural crest cell	Present	1A beta1 neo/+ s	Fully colonized enteric neural crest cells, similar as in the wild-type.

1A beta1 neo/+ si ©

Specimen 1A beta1 neo/+ si: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A beta1 neo/+ si	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A beta1 neo/+ si	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: small intestine	migratory enteric neural crest cell	Present	1A beta1 neo/+ si	Fully colonized enteric neural crest cells, similar as in the wild-type.

1A beta1 neo/+ ce ©

Specimen 1A beta1 neo/+ ce: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: cecum	migratory enteric neural crest cell	Present	1A beta1 neo/+ ce	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A beta1 neo/+ ce	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A beta1 neo/+ ce	Fully colonized enteric neural crest cells, similar as in the wild-type.

1A beta1 neo/+ co ©

Specimen 1A beta1 neo/+ co: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	1A beta1 neo/+ co	Fully colonized enteric neural crest cells in the colon, similar as in the wild-type.
TS21: large intestine	migratory enteric neural crest cell	Present	Regionally restricted	1A beta1 neo/+ co	Fully colonized enteric neural crest cells in the colon, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	1A beta1 neo/+ co	Fully colonized enteric neural crest cells in the colon, similar as in the wild-type.

1A Sox10 lacZ/+ s ©

Specimen 1A Sox10 lacZ/+ s: embryonic day 13.5; Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A Sox10 lacZ/+ s	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A Sox10 lacZ/+ s	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: stomach	migratory enteric neural crest cell	Present	1A Sox10 lacZ/+ s	Fully colonized enteric neural crest cells, similar as in the wild-type.

1A Sox10 lacZ/+ si ©

Specimen 1A Sox10 lacZ/+ si: embryonic day 13.5; Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A Sox10 lacZ/+ si	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A Sox10 lacZ/+ si	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: small intestine	migratory enteric neural crest cell	Present	1A Sox10 lacZ/+ si	Fully colonized enteric neural crest cells, similar as in the wild-type.

1A Sox10 lacZ/+ ce ©

Specimen 1A Sox10 lacZ/+ ce: embryonic day 13.5; Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: cecum	migratory enteric neural crest cell	Ambiguous	1A Sox10 lacZ/+ ce	Trace or absent, presented with colonization delay.
TS21: enteric nervous system	migratory enteric neural crest cell	Ambiguous	1A Sox10 lacZ/+ ce	Trace or absent, presented with colonization delay.
TS21: migrating neural crest	migratory enteric neural crest cell	Ambiguous	1A Sox10 lacZ/+ ce	Trace or absent, presented with colonization delay.

1A Sox10 lacZ/+ co ©

Specimen 1A Sox10 lacZ/+ co: embryonic day 13.5; Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Ambiguous	1A Sox10 lacZ/+ co	Trace or absent in the colon, presented with colonization delay.
TS21: large intestine	migratory enteric neural crest cell	Ambiguous	1A Sox10 lacZ/+ co	Trace or absent in the colon, presented with colonization delay.
TS21: migrating neural crest	migratory enteric neural crest cell	Ambiguous	1A Sox10 lacZ/+ co	Trace or absent in the colon, presented with colonization delay.

1A DH s ©

Specimen 1A DH s: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A DH s	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A DH s	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: stomach	migratory enteric neural crest cell	Present	1A DH s	Fully colonized enteric neural crest cells, similar as in the wild-type.

1A DH si ©

Specimen 1A DH si: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	1A DH si	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	1A DH si	Fully colonized enteric neural crest cells, similar as in the wild-type.
TS21: small intestine	migratory enteric neural crest cell	Present	1A DH si	Fully colonized enteric neural crest cells, similar as in the wild-type.

1A DH ce ©

Specimen 1A DH ce: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS21: cecum	migratory enteric neural crest cell	Ambiguous	1A DH ce	Trace or absent, presented with colonization delay.
TS21: enteric nervous system	migratory enteric neural crest cell	Ambiguous	1A DH ce	Trace or absent, presented with colonization delay.
TS21: migrating neural crest	migratory enteric neural crest cell	Ambiguous	1A DH ce	Trace or absent, presented with colonization delay.

1A DH co ©

Specimen 1A DH co: embryonic day 13.5; Itgb1^tm2Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS21: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	1A DH co	Trace or absent in the colon, presented with colonization delay.
TS21: large intestine	migratory enteric neural crest cell	Present	Regionally restricted	1A DH co	Trace or absent in the colon, presented with colonization delay.
TS21: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	1A DH co	Trace or absent in the colon, presented with colonization delay.

2A controls s ©

Specimen 2A controls s: embryonic day 14.5 (more

)
Note: control; three possible genotypes; possibilities detailed in Table 1.

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A controls s
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A controls s
TS22: stomach	migratory enteric neural crest cell	Present	2A controls s

2A controls si-p ©

Specimen 2A controls si-p: embryonic day 14.5 (more

)
Note: proximal part of the small intestine

Structure	Level	Image
TS22: enteric nervous system	Present	2A controls si-p
TS22: migrating neural crest	Present	2A controls si-p
TS22: small intestine	Present	2A controls si-p

2A controls si-m ©

Specimen 2A controls si-m: embryonic day 14.5 (more

)
Note: median part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A controls si-m
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A controls si-m
TS22: small intestine	migratory enteric neural crest cell	Present	2A controls si-m

2A controls ce ©

Specimen 2A controls ce: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Image
TS22: cecum	migratory enteric neural crest cell	Present	2A controls ce
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A controls ce
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A controls ce

2A controls co ©

Specimen 2A controls co: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	2A controls co	Fully colonized enteric neural crest cells in the colon.
TS22: large intestine	migratory enteric neural crest cell	Present	Regionally restricted	2A controls co	Fully colonized enteric neural crest cells in the colon.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	2A controls co	Fully colonized enteric neural crest cells in the colon.

2A Sox10 lacZ/+ s ©

Specimen 2A Sox10 lacZ/+ s: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ s
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ s
TS22: stomach	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ s

2A Sox10 lacZ/+ si-p ©

Specimen 2A Sox10 lacZ/+ si-p: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)
Note: proximal part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ si-p
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ si-p
TS22: small intestine	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ si-p

2A Sox10 lacZ/+ si-m ©

Specimen 2A Sox10 lacZ/+ si-m: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)
Note: median part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ si-m
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ si-m
TS22: small intestine	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ si-m

2A Sox10 lacZ/+ ce ©

Specimen 2A Sox10 lacZ/+ ce: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image
TS22: cecum	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ ce
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ ce
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A Sox10 lacZ/+ ce

2A Sox10 lacZ/+ co ©

Specimen 2A Sox10 lacZ/+ co: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	2A Sox10 lacZ/+ co	Colonization delay was seen. No positive cells from the middle of the colon caudally.
TS22: large intestine	migratory enteric neural crest cell	Present	Regionally restricted	2A Sox10 lacZ/+ co	Colonization delay was seen. No positive cells from the middle of the colon caudally.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	2A Sox10 lacZ/+ co	Colonization delay was seen. No positive cells from the middle of the colon caudally.

2A beta1-null s ©

Specimen 2A beta1-null s: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A beta1-null s
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A beta1-null s
TS22: stomach	migratory enteric neural crest cell	Present	2A beta1-null s

2A beta1-null si-p ©

Specimen 2A beta1-null si-p: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. proximal part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A beta1-null si-p
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A beta1-null si-p
TS22: small intestine	migratory enteric neural crest cell	Present	2A beta1-null si-p

2A beta1-null si-m ©

Specimen 2A beta1-null si-m: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. median part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A beta1-null si-m
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A beta1-null si-m
TS22: small intestine	migratory enteric neural crest cell	Present	2A beta1-null si-m

2A beta1-null ce ©

Specimen 2A beta1-null ce: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cecum	migratory enteric neural crest cell	Present	Regionally restricted	2A beta1-null ce	Colonization delay was seen. No positive cells from the caecum caudally.
TS22: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	2A beta1-null ce	Colonization delay was seen. No positive cells from the caecum caudally.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	2A beta1-null ce	Colonization delay was seen. No positive cells from the caecum caudally.

2A beta1-null co ©

Specimen 2A beta1-null co: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	2A beta1-null co	Colonization delay was seen. No positive cells in the colon.
TS22: large intestine	migratory enteric neural crest cell	Present	Regionally restricted	2A beta1-null co	Colonization delay was seen. No positive cells in the colon.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	2A beta1-null co	Colonization delay was seen. No positive cells in the colon.

2A DH s ©

Specimen 2A DH s: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: DH: double heterozygous; two possible genotypes; detailed in Table 1.

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A DH s
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A DH s
TS22: stomach	migratory enteric neural crest cell	Present	2A DH s

2A DH si-p ©

Specimen 2A DH si-p: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: proximal part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A DH si-p
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A DH si-p
TS22: small intestine	migratory enteric neural crest cell	Present	2A DH si-p

2A DH si-m ©

Specimen 2A DH si-m: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: median part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A DH si-m
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A DH si-m
TS22: small intestine	migratory enteric neural crest cell	Present	2A DH si-m

2A DH ce ©

Specimen 2A DH ce: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS22: cecum	migratory enteric neural crest cell	Ambiguous	2A DH ce	Three of the seven embryos had a slight colonization delay compared to single mutants.
TS22: enteric nervous system	migratory enteric neural crest cell	Ambiguous	2A DH ce	Three of the seven embryos had a slight colonization delay compared to single mutants.
TS22: migrating neural crest	migratory enteric neural crest cell	Ambiguous	2A DH ce	Three of the seven embryos had a slight colonization delay compared to single mutants.

2A DH co ©

Specimen 2A DH co: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Ambiguous	Regionally restricted	2A DH co	Three of the seven DH embryos had a slight colonization delay compared to single mutants.
TS22: large intestine	migratory enteric neural crest cell	Ambiguous	Regionally restricted	2A DH co	Three of the seven DH embryos had a slight colonization delay compared to single mutants.
TS22: migrating neural crest	migratory enteric neural crest cell	Ambiguous	Regionally restricted	2A DH co	Three of the seven DH embryos had a slight colonization delay compared to single mutants.

2A DM s ©

Specimen 2A DM s: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A DM s
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A DM s
TS22: stomach	migratory enteric neural crest cell	Present	2A DM s

2A DM si-p ©

Specimen 2A DM si-p: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. proximal part of the small intestine

Structure	Cell Type	Level	Image
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2A DM si-p
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2A DM si-p
TS22: small intestine	migratory enteric neural crest cell	Present	2A DM si-p

2A DM si-m ©

Specimen 2A DM si-m: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. median part of the small intestine

Structure	Cell Type	Level	Pattern	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	2A DM si-m	Severe colonization delay was seen. Staining stopped within the first half of the small intestine of all embryos analyzed.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	2A DM si-m	Severe colonization delay was seen. Staining stopped within the first half of the small intestine of all embryos analyzed.
TS22: small intestine	migratory enteric neural crest cell	Present	Regionally restricted	2A DM si-m	Severe colonization delay was seen. Staining stopped within the first half of the small intestine of all embryos analyzed.

2A DM ce ©

Specimen 2A DM ce: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cecum	migratory enteric neural crest cell	Absent		2A DM ce

2A DM co ©

Specimen 2A DM co: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: large intestine	migratory enteric neural crest cell	Absent		2A DM co	Severe colonization delay was seen. Staining stopped within the first half of the small intestine of all embryos analyzed.

2C left ©

Specimen 2C Sox10 lacZ/+: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2C left	Normal neuronal network as in the wild-type.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2C left	Normal neuronal network as in the wild-type.
TS22: small intestine	migratory enteric neural crest cell	Present	2C left	Normal neuronal network as in the wild-type.

2C middle ©

Specimen 2C beta1-null: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2C middle	Disorganization of the neuronal network with abnormal aggregates surrounded by enlarged negative spaces was seen.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2C middle	Disorganization of the neuronal network with abnormal aggregates surrounded by enlarged negative spaces was seen.
TS22: small intestine	migratory enteric neural crest cell	Present	2C middle	Disorganization of the neuronal network with abnormal aggregates surrounded by enlarged negative spaces was seen.

2C right ©

Specimen 2C DM: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	2C right	Disorganization of the neuronal network with abnormal aggregates surrounded by enlarged negative spaces was exacerbated. Negative spaces were larger than in beta1-null mutant.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	2C right	Disorganization of the neuronal network with abnormal aggregates surrounded by enlarged negative spaces was exacerbated. Negative spaces were larger than in beta1-null mutant.
TS22: small intestine	migratory enteric neural crest cell	Present	2C right	Disorganization of the neuronal network with abnormal aggregates surrounded by enlarged negative spaces was exacerbated. Negative spaces were larger than in beta1-null mutant.

3A Controls si-p ©

Specimen 3A controls si-p: embryonic day 14.5 (more

)
Note: Control; three possible genotypes; possibilities detailed in Table 1. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A Controls si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A Controls si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A Controls si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

3A Controls si-m ©

Specimen 3A controls si-m: embryonic day 14.5 (more

)
Note: Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A Controls si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A Controls si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A Controls si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

Specimen 3A controls mfv: embryonic day 14.5 (more

)
Note: Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A Controls mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A Controls mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A Controls mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

Specimen 3A beta1-null si-p: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A beta1-null si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A beta1-null si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A beta1-null si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

Specimen 3A beta1-null si-m: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A beta1-null si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A beta1-null si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A beta1-null si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

Specimen 3A beta1-null mfv: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A beta1-null mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A beta1-null mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A beta1-null mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

3A DH si-p ©

Specimen 3A DH si-p: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: DH: double heterozygous; two possible genotypes; detailed in Table 1. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A DH si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A DH si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A DH si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

3A DH si-m ©

Specimen 3A DH si-m: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A DH si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A DH si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A DH si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

3A DH mfv ©

Specimen 3A DH mfv: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A DH mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A DH mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A DH mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

3A DM si-p ©

Specimen 3A DM si-p: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A DM si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells), with enlarged negative spaces compared to other genotypes.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A DM si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells), with enlarged negative spaces compared to other genotypes.
TS22: small intestine	migratory enteric neural crest cell	Present	3A DM si-p	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells), with enlarged negative spaces compared to other genotypes.

3A DM si-m ©

Specimen 3A DM si-m: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A DM si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells), with enlarged negative spaces compared to other genotypes.
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A DM si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells), with enlarged negative spaces compared to other genotypes.
TS22: small intestine	migratory enteric neural crest cell	Present	3A DM si-m	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells), with enlarged negative spaces compared to other genotypes.

3A DM mfv ©

Specimen 3A DM mfv: embryonic day 14.5; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant. Double labeled: red - Tubb3; green - PLAT-cre lineage.

Structure	Cell Type	Level	Image	Note
TS22: enteric nervous system	migratory enteric neural crest cell	Present	3A DM mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: migrating neural crest	migratory enteric neural crest cell	Present	3A DM mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).
TS22: small intestine	migratory enteric neural crest cell	Present	3A DM mfv	Positive cells exhibited a similar pattern with YFP+ cells (enteric neural crest cells).

Specimen 4B controls: embryonic day 12.25 (more

)
Note: control; three possible genotypes; possibilities detailed in Table 1.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cecum	migratory enteric neural crest cell	Present		4B Tuj1 Controls
TS20: enteric nervous system	migratory enteric neural crest cell	Present		4B Tuj1 Controls
TS20: migrating neural crest	migratory enteric neural crest cell	Present		4B Tuj1 Controls
TS20: small intestine	migratory enteric neural crest cell	Present		4B Tuj1 Controls
TS20: stomach	migratory enteric neural crest cell	Present		4B Tuj1 Controls
TS20: large intestine	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 Controls	Positive cells moving in lines to colonize the colon.

Specimen 4B Sox10 lacZ/+: embryonic day 12.25; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 Sox10 lacZ/+	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping in the final quarter of the midgut.
TS20: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 Sox10 lacZ/+	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping in the final quarter of the midgut.
TS20: small intestine	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 Sox10 lacZ/+	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping in the final quarter of the midgut.
TS20: stomach	migratory enteric neural crest cell	Present		4B Tuj1 Sox10 lacZ/+
TS20: cecum		Absent		4B Tuj1 Sox10 lacZ/+
TS20: large intestine		Absent		4B Tuj1 Sox10 lacZ/+

Specimen 4B Sox10 beta1-null: embryonic day 12.25; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 beta1-null	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping just before the caecum.
TS20: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 beta1-null	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping just before the caecum.
TS20: small intestine	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 beta1-null	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping just before the caecum.
TS20: stomach	migratory enteric neural crest cell	Present		4B Tuj1 beta1-null
TS20: cecum	migratory enteric neural crest cell	Absent		4B Tuj1 beta1-null
TS20: large intestine	migratory enteric neural crest cell	Absent		4B Tuj1 beta1-null

4B Tuj1 DH ©

Specimen 4B DH: embryonic day 12.25; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: DH: double heterozygous; two possible genotypes; detailed in Table 1.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 DH	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping just before the caecum.
TS20: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 DH	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping just before the caecum.
TS20: small intestine	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 DH	Slight delay in colonization compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping just before the caecum.
TS20: stomach	migratory enteric neural crest cell	Present		4B Tuj1 DH
TS20: cecum	migratory enteric neural crest cell	Absent		4B Tuj1 DH
TS20: large intestine	migratory enteric neural crest cell	Absent		4B Tuj1 DH

4B Tuj1 DM ©

Specimen 4B DM: embryonic day 12.25; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: enteric nervous system	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 DM	Colonization delay and network disorganization were seen compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping before the second half of the midgut.
TS20: migrating neural crest	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 DM	Colonization delay and network disorganization were seen compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping before the second half of the midgut.
TS20: small intestine	migratory enteric neural crest cell	Present	Regionally restricted	4B Tuj1 DM	Colonization delay and network disorganization were seen compared to controls, with the front of migrating enteric neural crest cells in the mutants stopping before the second half of the midgut.
TS20: stomach	migratory enteric neural crest cell	Present		4B Tuj1 DM
TS20: large intestine	migratory enteric neural crest cell	Absent		4B Tuj1 DM

5A Controls ©

Specimen 5A controls: postnatal newborn (more

)
Note: control; three possible genotypes; possibilities detailed in Table 1.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: enteric nervous system	enteric neuron	Present		5A Controls
TS27: small intestine	enteric neuron	Present		5A Controls

Specimen 5A Sox10 lacZ/+: postnatal newborn; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1⁺, Sox10^tm1Weg/Sox10⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: enteric nervous system	enteric neuron	Present		5A Sox10 lacZ/+	The network was similar as in the controls.
TS27: small intestine	enteric neuron	Present		5A Sox10 lacZ/+	The network was similar as in the controls.

Specimen 5A beta1-null: postnatal newborn; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: enteric nervous system	enteric neuron	Present		5A beta1-null	The network was similar as in the controls.
TS27: small intestine	enteric neuron	Present		5A beta1-null	The network was similar as in the controls.

5A DH ©

Specimen 5A DH: postnatal newborn; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺ (more

)
Note: DH: double heterozygous; two possible genotypes; detailed in Table 1.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: enteric nervous system	enteric neuron	Present		5A DH	The network was similar as in the controls.
TS27: small intestine	enteric neuron	Present		5A DH	The network was similar as in the controls.

5A DM ©

Specimen 5A DM: postnatal newborn; Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor⁺, Itgb1^tm1Ref/Itgb1^tm2Ref, Sox10^tm1Weg/Sox10⁺, Tg(PLAT-cre)116Sdu/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: enteric nervous system	enteric neuron	Present		5A DM	The network was more disorganized in colonized regions compared to other genotypes.
TS27: small intestine	enteric neuron	Present		5A DM	The network was more disorganized in colonized regions compared to other genotypes.

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