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Gene Expression Data
Assay Details
Assay
Reference: J:336140 Yokomizo T, et al., Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature. 2022 Sep;609(7928):779-784
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7489681
Gene symbol: Hlf
Gene name: hepatic leukemia factor
Reporter: tdTomato
Direct Detection
Results
Specimen ED2b: embryonic day 10.5; Hlfem1Suda/Hlf+ (more )
Note: Triple labeled: red - Hlf reporter; green - Kit (assay MGI:7489679); white - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED2b (3D) , ED2b inset tdTomato , ED2b inset (merge) , ED2b (optical section) Expression in hematopoietic clusters.
TS17: dorsal aorta endothelium Absent ED2b (optical section) , ED2b inset tdTomato , ED2b (3D) , ED2b inset (merge)
Specimen ED2c: embryonic day 10.5; Hlfem1Suda/Hlf+ (more )
Note: Triple labeled: red - Hlf reporter; green - Kit (assay MGI:7489679); white - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED2c tdTomato , ED2c (merge) Expression in hematopoietic clusters.
TS17: yolk sac blood vessel Absent ED2c tdTomato , ED2c (merge)
Specimen ED7g AGM: embryonic day 10.5; Hlfem1Suda/Hlf+, Mecomtm2Miku/Mecom+ (more )
Note: Double labeled: red - Hlf reporter; green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED7g AGM tdTomato , ED7g AGM Merge Expression in hematopoietic clusters.
TS17: dorsal aorta endothelium Absent ED7g AGM Merge , ED7g AGM tdTomato
Specimen ED7g Yolk sac: embryonic day 10.5; Hlfem1Suda/Hlf+, Mecomtm2Miku/Mecom+ (more )
Note: Double labeled: red - Hlf reporter; green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED7g Yolk sac Merge , ED7g Yolk sac tdTomato Expression in hematopoietic clusters.
TS17: yolk sac blood vessel Absent ED7g Yolk sac tdTomato , ED7g Yolk sac Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory