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Gene Expression Data
Assay Details
Assay
Reference: J:336140 Yokomizo T, et al., Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature. 2022 Sep;609(7928):779-784
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7489680
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody
Assay notes: Two Pecam1 antibodies were described, but the one used in this assay was not detailed. Anti-CD31 (2H8) ( MGI:3704881 may have been used in this assay.
Results
Specimen 2c AGM: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Triple labeled: white - Pecam1; red - Kit (assay MGI:7489679); green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: dorsal aorta endothelium Present 2c AGM (merge)
TS17: hematopoietic system Present Regionally restricted 2c AGM (merge) Expression in hematopoietic clusters.
Specimen 2c Yolk sac: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Triple labeled: white - Pecam1; red - Kit (assay MGI:7489679); green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present 2c Yolk sac (merge)
TS17: hematopoietic system Present Regionally restricted 2c Yolk sac (merge) Expression in hematopoietic clusters.
Specimen ED2b: embryonic day 10.5; Hlfem1Suda/Hlf+ (more )
Note: Triple labeled: white - Pecam1; red - Hlf reporter (assay MGI:7489681); green - Kit (assay MGI:7489679).
Structure Level Pattern Image Note
TS17: dorsal aorta endothelium Present ED2b (3D) , ED2b inset (merge) , ED2b (optical section) , ED2b inset CD31
TS17: hematopoietic system Present Regionally restricted ED2b (optical section) , ED2b (3D) , ED2b inset c-Kit , ED2b inset (merge) Expression in hematopoietic clusters.
Specimen ED2c: embryonic day 10.5; Hlfem1Suda/Hlf+ (more )
Note: Triple labeled: white - Pecam1; red - Hlf reporter (assay MGI:7489681); green - Kit (assay MGI:7489679).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present ED2c (merge)
TS17: hematopoietic system Present Regionally restricted ED2c (merge) Expression in hematopoietic clusters.
Specimen ED5a Embryo proper: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Double labeled: white - Pecam1; green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: dorsal aorta Present ED5a Embryo (merge)
TS17: umbilical artery Present ED5a Embryo (merge)
TS17: vitelline artery Present ED5a Embryo (merge)
Specimen ED5a Yolk sac: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Double labeled: white - Pecam1; green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: yolk sac blood vessel Present ED5a Yolk sac (merge)
Specimen ED5b Umbilical artery: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Double labeled: white - Pecam1; green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: umbilical artery Present ED5b Umbilical artery merge
Specimen ED5b Vitelline artery: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Double labeled: white - Pecam1; green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: vitelline artery Present ED5b Vitelline artery merge
Specimen ED5b Umbilical vein: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Double labeled: white - Pecam1; green - Mecom reporter (assay MGI:7489674).
Structure Level Pattern Image Note
TS17: umbilical vein Present ED5b Umbilical vein merge
Specimen ED8b Evi1+/+: embryonic day 10.5 (more )
Note: Double labeled: magenta - Pecam1; green - Kit (assay MGI:7489679).
Structure Level Pattern Image Note
TS17: dorsal aorta Present ED8b Evi1+/+
Specimen Ed8bEvi1+/-: embryonic day 10.5; Mecomtm1.1Miku/Mecom+ (more )
Note: Double labeled: magenta - Pecam1; green - Kit (assay MGI:7489679).
Structure Level Pattern Image Note
TS17: dorsal aorta Present ED8b Evi1+/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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