Hlf^em1Suda
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hlf^em1Suda
• Name: hepatic leukemia factor; endonuclease-mediated mutation 1, Toshio Suda
• MGI ID: MGI:7429022
• Synonyms: Hlf^tdTomato
• Gene: Hlf Location: Chr11:90227362-90281721 bp, - strand Genetic Position: Chr11, 55.03 cM
• Alliance: Hlf^em1Suda page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: The sequence encoding T2A-tdTomato was inserted before the endogenous stop codon within exon 4 via CRISPR/Cas9 technology. Western blot analysis confirmed normal level of protein expression. Blood cell analysis shows normal hematopoietic differentiation in adult heterozygotes. (J:327106)

Expression

In Mice Carrying this Mutation:

14 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hlf Mutation: 16 strains or lines available

References

• Original: J:327106 Yokomizo T, et al., Hlf marks the developmental pathway for hematopoietic stem cells but not for erythro-myeloid progenitors. J Exp Med. 2019 Jul 1;216(7):1599-1614
• All: 5 reference(s)