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Gene Expression Data
Assay Details
Assay
Reference: J:336140 Yokomizo T, et al., Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature. 2022 Sep;609(7928):779-784
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7489679
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-KIT (553353)
Detection system: Secondary antibody
Assay notes: Two Kit antibodies were described, but the one used in this assay was not detailed. Anti-Kit MGI:6715334 may have been used in this assay.
Results
Specimen 2c AGM: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Triple labeled: red - Kit; green - Mecom reporter (assay MGI:7489674); white - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted 2c AGM (merge) Expression in hematopoietic clusters.

Specimen 2c Yolk sac: embryonic day 10.5; Mecomtm2Miku/Mecom+ (more )
Note: Triple labeled: red - Kit; green - Mecom reporter (assay MGI:7489674); white - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted 2c Yolk sac (merge) Expression in hematopoietic clusters.

Specimen ED2b: embryonic day 10.5; Hlfem1Suda/Hlf+ (more )
Note: Triple labeled: green - Kit; red - Hlf reporter (assay MGI:7489681); white - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED2b (3D) , ED2b (optical section) , ED2b inset c-Kit , ED2b inset (merge) Expression in hematopoietic clusters.

Specimen ED2c: embryonic day 10.5; Hlfem1Suda/Hlf+ (more )
Note: Triple labeled: green - Kit; red - Hlf reporter (assay MGI:7489681); white - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED2c (merge) , ED2c c-Kit Expression in hematopoietic clusters.

Specimen ED8b Evi1+/+: embryonic day 10.5 (more )
Note: Double labeled: green - Kit; magenta - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED8b Evi1+/+ Expression in hematopoietic clusters.

Specimen Ed8bEvi1+/-: embryonic day 10.5; Mecomtm1.1Miku/Mecom+ (more )
Note: Double labeled: green - Kit; magenta - Pecam1 (assay MGI:7489680).
Structure Level Pattern Image Note
TS17: hematopoietic system Present Regionally restricted ED8b Evi1+/- Expression in hematopoietic clusters.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory