Gene Expression Data
Assay Details
Assay
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Reference:
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J:336140
Yokomizo T, et al., Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature. 2022 Sep;609(7928):779-784
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7489679
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Gene symbol:
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Kit
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Gene name:
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KIT proto-oncogene receptor tyrosine kinase
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Antibody:
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anti-KIT (553353)
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Detection system:
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Secondary antibody
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Assay notes:
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Two Kit antibodies were described, but the one used in this assay was not detailed. Anti-Kit MGI:6715334 may have been used in this assay.
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Results
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Specimen
2c AGM:
embryonic day 10.5; Mecomtm2Miku/Mecom +
(more )
Note: Triple labeled: red - Kit; green - Mecom reporter (assay MGI:7489674); white - Pecam1 (assay MGI:7489680).
Specimen
2c AGM:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Mecomtm2Miku/Mecom+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: red - Kit; green - Mecom reporter (assay MGI:7489674); white - Pecam1 (assay MGI:7489680).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hematopoietic system |
Present |
Regionally restricted |
2c AGM (merge)
|
Expression in hematopoietic clusters.
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Specimen
2c Yolk sac:
embryonic day 10.5; Mecomtm2Miku/Mecom +
(more )
Note: Triple labeled: red - Kit; green - Mecom reporter (assay MGI:7489674); white - Pecam1 (assay MGI:7489680).
Specimen
2c Yolk sac:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Mutant Allele(s):
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Mecomtm2Miku/Mecom+
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Age:
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embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
|
Note:
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Triple labeled: red - Kit; green - Mecom reporter (assay MGI:7489674); white - Pecam1 (assay MGI:7489680).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hematopoietic system |
Present |
Regionally restricted |
2c Yolk sac (merge)
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Expression in hematopoietic clusters.
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Specimen
ED2b:
embryonic day 10.5; Hlfem1Suda/Hlf +
(more )
Note: Triple labeled: green - Kit; red - Hlf reporter (assay MGI:7489681); white - Pecam1 (assay MGI:7489680).
Specimen
ED2b:
(close )
Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Hlfem1Suda/Hlf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
|
Note:
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Triple labeled: green - Kit; red - Hlf reporter (assay MGI:7489681); white - Pecam1 (assay MGI:7489680).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hematopoietic system |
Present |
Regionally restricted |
ED2b (3D)
,
ED2b (optical section)
,
ED2b inset c-Kit
,
ED2b inset (merge)
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Expression in hematopoietic clusters.
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Specimen
ED2c:
embryonic day 10.5; Hlfem1Suda/Hlf +
(more )
Note: Triple labeled: green - Kit; red - Hlf reporter (assay MGI:7489681); white - Pecam1 (assay MGI:7489680).
Specimen
ED2c:
(close )
Genetic Background:
|
involves: C57BL/6
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Mutant Allele(s):
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Hlfem1Suda/Hlf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: green - Kit; red - Hlf reporter (assay MGI:7489681); white - Pecam1 (assay MGI:7489680).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hematopoietic system |
Present |
Regionally restricted |
ED2c (merge)
,
ED2c c-Kit
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Expression in hematopoietic clusters.
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Specimen
ED8b Evi1+/+:
embryonic day 10.5
(more )
Note: Double labeled: green - Kit; magenta - Pecam1 (assay MGI:7489680).
Specimen
ED8b Evi1+/+:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
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2% Paraformaldehyde
|
Note:
|
Double labeled: green - Kit; magenta - Pecam1 (assay MGI:7489680).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hematopoietic system |
Present |
Regionally restricted |
ED8b Evi1+/+
|
Expression in hematopoietic clusters.
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Specimen
Ed8bEvi1+/-:
embryonic day 10.5; Mecomtm1.1Miku/Mecom +
(more )
Note: Double labeled: green - Kit; magenta - Pecam1 (assay MGI:7489680).
Specimen
Ed8bEvi1+/-:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Mecomtm1.1Miku/Mecom+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
|
Note:
|
Double labeled: green - Kit; magenta - Pecam1 (assay MGI:7489680).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hematopoietic system |
Present |
Regionally restricted |
ED8b Evi1+/-
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Expression in hematopoietic clusters.
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