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Gene Expression Data
Assay Details
Assay
Reference: J:326689 Phan TP, et al., Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev. 2022 Jun 1;36(11-12):718-736
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7442686
Gene symbol: Tubg2
Gene name: tubulin, gamma 2
Antibody: anti-gamma-tubulin
Detection system: Secondary antibody coupled to Alexa Fluor 647
Assay notes: The specificity of the antibody/probe used was not detailed; both family members have been annotated.
Results
Specimen 2C +/+: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: intestine lamina propria plasma cell Present 2C +/+ Expressed in the pericentriolar material.
Specimen 2C +/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: intestine lamina propria plasma cell Present 2C +/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2C deltau/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: intestine lamina propria plasma cell Present 2C deltau/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2D +/+: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: epidermis keratinocyte Present 2D +/+ Expressed in the pericentriolar material.
Specimen 2D +/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: epidermis keratinocyte Present 2D +/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2D deltau/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: epidermis keratinocyte Present 2D deltau/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2E +/+: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: spleen splenocyte Present 2E +/+ Expressed in the pericentriolar material.
Specimen 2E +/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: spleen splenocyte Present 2E +/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2E deltau/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: spleen splenocyte Present 2E deltau/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 4C +/+: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Pou5f1 (assay MGI:7442648).
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C +/+
Specimen 4C deltau/deltau: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Pou5f1 (assay MGI:7442648).
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C deltau/deltau Similar as in the wild-type. A small fraction had an increased number of centrin-GFP foci that were often observed in a "flower-like" pattern.
Specimen 4G +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G merge +/+
Specimen 4G +/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G merge +/deltau An increased number of centrin-GFP foci was seen.
Specimen 4G deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G merge deltau/deltau An increased number of centrin-GFP foci was seen.
Specimen 5K +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K +/+ lower , 5K +/+ upper
Specimen 5K deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K deltau/deltau upper , 5K deltau/deltau lower An increased number of centrin-GFP foci was seen.
Specimen 5K deltau/-: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em2.1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K deltau/- upper , 5K deltau/- lower No longer display centriole amplification.
Specimen S4D +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present S4D +/+ lower , S4D +/+ upper
Specimen S4D deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present S4D deltau/deltau lower , S4D deltau/deltau upper Some positive cells with supernumerary centrioles exhibited multipolar mitotic spindles, suggesting that these cells could generate aneuploid daughter cells with micronuclei or abnormal nuclear shape.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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