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Gene Expression Data
Assay Details
Assay
Reference: J:229936 Cano E, et al., Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):656-61
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7431230
Gene symbol: Wt1
Gene name: WT1 transcription factor
Reporter: GFP
Antibody: anti-GFP (ab13970)
Detection system: Secondary antibody
Results
Specimen 2A: embryonic day 10.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: green - Wt1 reporter; red - Wt1 protein (assay MGI:7431136).
Structure Level Pattern Image Note
TS17: visceral pericardium Present Ubiquitous 2A
Specimen 2B: embryonic day 12.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: green - Wt1 reporter; red - Wt1 protein (assay MGI:7431136).
Structure Level Pattern Image Note
TS20: visceral pericardium Present 2B
TS20: heart Present Regionally restricted 2B Expressed in epicardial-derived cells, which accumulate at the ventricular, atrio-ventricular, and interventricular subepicardium.
Specimen 2C: embryonic day 11.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: green - Wt1 reporter; red - Pecam1 (assay MGI:7431138).
Structure Level Pattern Image Note
TS19: visceral pericardium Present 2C
Specimen 2D: embryonic day 12.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: green - Wt1 reporter; red - Pecam1 (assay MGI:7431138).
Structure Level Pattern Image Note
TS20: coronary vascular plexus Present 2D Coexpressed with Pecam1 in subepicardial and intramyocardial cells of the coronary vasculature.
Specimen 2E/E": embryonic day 12.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: green - Wt1 reporter; red - Pecam1 (assay MGI:7431138).
Structure Level Pattern Image Note
TS20: coronary vascular plexus Present 2E" , 2E Coexpressed with Pecam1 in subepicardial coronary vasculature.
Specimen 2F: embryonic day 13.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Triple labeled: green - Wt1 reporter; blue - Acta2 (assay MGI:7431145); red - Pecam1 (assay MGI:7431138).
Structure Level Pattern Image Note
TS21: coronary artery Present 2F Coexpressed with Pecam1 in intramyocardial coronary vasculature. Some positive cells displayed the typical spindle-shaped morphology of migratory mesenchymal cells, with their major axis oriented orthogonally with respect to the epicardial surface.
Specimen 2G: embryonic day 14.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Triple labeled: green - Wt1 reporter; blue - Acta2 (assay MGI:7431145); red - Pecam1 (assay MGI:7431138).
Structure Level Pattern Image Note
TS22: coronary artery Present 2G Coexpressed with Pecam1 in a few cells in intramyocardial coronary vasculature.
Specimen 2H: embryonic day 18.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: green - Wt1 reporter; red - Pecam1 (assay MGI:7431138).
Structure Level Pattern Image Note
TS26: coronary artery Present 2H Coexpressed with Pecam1 in fewer cells compared with earlier stages.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory