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Gene Expression Data
Assay Details
Assay
Reference: J:229936 Cano E, et al., Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):656-61
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7431136
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: 6F-H2 (M3561)
Detection system: Secondary antibody
Results
Specimen 1D: embryonic day 9.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Wt1; green - Cre reporter
Structure Level Pattern Image Note
TS15: visceral pericardium Present Ubiquitous 1D
TS15: proepicardium Present 1D
TS15: septum transversum Present 1D
Specimen 1E: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Wt1; green - Cre reporter
Structure Level Pattern Image Note
TS19: septum transversum Present 1E
TS19: visceral pericardium Present 1E
Specimen 1F: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Wt1; green - Cre reporter
Structure Level Pattern Image Note
TS19: visceral pericardium Present 1F
Specimen 1F': embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Wt1; green - Cre reporter
Structure Level Pattern Image Note
TS19: visceral pericardium Present 1F'
TS19: heart Present Regionally restricted 1F' Expressed in a few epicardial-derived cells, which migrated from the subepicardium into the myocardial layers.
Specimen 2A: embryonic day 10.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: red - Wt1 protein; green - Wt1 reporter (assay MGI:7431230).
Structure Level Pattern Image Note
TS17: visceral pericardium Present Ubiquitous 2A
Specimen 2B: embryonic day 12.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: red - Wt1 protein; green - Wt1 reporter (assay MGI:7431230).
Structure Level Pattern Image Note
TS20: visceral pericardium Present 2B
TS20: heart Present Regionally restricted 2B Expressed in epicardial-derived cells, which accumulate at the ventricular, atrio-ventricular, and interventricular subepicardium.
Specimen S4A: embryonic day 12.5; Wt1tm1.1Ndha/Wt1tm1.1Ndha (more )
Structure Level Pattern Image Note
TS20: visceral pericardium Present S4A
Specimen S4C: embryonic day 12.5; Wt1tm1.1Ndha/Wt1tm1.1Ndha, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: visceral pericardium Absent S4C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory