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Gene Expression Data
Assay Details
Assay
Reference: J:260124 Goldmann T, et al., Origin, fate and dynamics of macrophages at central nervous system interfaces. Nat Immunol. 2016 Jul;17(7):797-805
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7382874
Gene symbol: Aif1
Gene name: allograft inflammatory factor 1
Antibody: anti-Iba1 (019-19741)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3f Meninges Cx3cr1GFP/WT: embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red - Aif1; green - Cx3cr1 (assay MGI:7382870).
Structure Cell Type Level Pattern Image Note
TS22: meninges meningeal macrophage Present 3f Meninges Cx3cr1GFP/WT GFP and Aif1 expression co-localized in meningeal macrophages.
Specimen 3f Choroid plexus Cx3cr1GFP/WT: embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red - Aif1; green - Cx3cr1 (assay MGI:7382870).
Structure Cell Type Level Pattern Image Note
TS22: choroid plexus epithelium choroid plexus macrophage Present 3f Choriod plexus Cx3cr1GFP/WT GFP and Aif1 expression co-localized in choroid plexus macrophages.
TS22: choroid plexus stroma choroid plexus macrophage Present 3f Choriod plexus Cx3cr1GFP/WT GFP and Aif1 expression co-localized in choroid plexus macrophages.
Specimen 3f Meninges Irf8-/-;Cx3cr1GFP/wt: embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1+, Irf8tm1Hor/Irf8tm1Hor (more )
Note: Double labeled: red - Aif1; green - Cx3cr1 (assay MGI:7382870).
Structure Cell Type Level Pattern Image Note
TS22: meninges meningeal macrophage Present 3f Meninges Irf8-/- Cx3cr1GFP/WT There was a reduction in the number of meningeal macrophages noted in the Irf8 mutant background.
Specimen 3f Choroid plexus Irf8-/-;Cx3cr1GFP/wt: embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1+, Irf8tm1Hor/Irf8tm1Hor (more )
Note: Double labeled: red - Aif1; green - Cx3cr1 (assay MGI:7382870).
Structure Cell Type Level Pattern Image Note
TS22: choroid plexus epithelium choroid plexus macrophage Present 3f Choriod plexus Irf8-/- Cx3cr1GFP/WT Choroid plexus macrophages were not affected in the Irf8 mutant background.
TS22: choroid plexus stroma choroid plexus macrophage Present 3f Choriod plexus Irf8-/- Cx3cr1GFP/WT Choroid plexus macrophages were not affected in the Irf8 mutant background.
Specimen 6b Meninges: postnatal; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Flt3-cre)#Ccb/0 (more )
Note: Conditional mutant. Double labeled: red - Aif1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS28: meninges meningeal macrophage Present 6b Meninges
Specimen 6b Perivascular space: postnatal; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Flt3-cre)#Ccb/0 (more )
Note: Conditional mutant. Double labeled: red - Aif1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS28: brain perivascular macrophage Present 6b Perivascular space
Specimen 6b Choroid plexus: postnatal; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Flt3-cre)#Ccb/0 (more )
Note: Conditional mutant. Double labeled: red - Aif1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS28: choroid plexus epithelium choroid plexus macrophage Present 6b Choroid plexus
TS28: choroid plexus stroma choroid plexus macrophage Present 6b Choroid plexus
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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