Cx3cr1^tm1Litt
Targeted Allele Detail

Summary

• Symbol: Cx3cr1^tm1Litt
• Name: C-X3-C motif chemokine receptor 1; targeted mutation 1, Dan R Littman
• MGI ID: MGI:2670351
• Synonyms: CX3CR1^-, CX3CR1^EGFP, CX3CR1-EGFP, Cx3cr1^gfp, CX3CR1^GFP, CX3CR1-GFP, CX3CR-GFP
• Gene: Cx3cr1 Location: Chr9:119877749-119897362 bp, - strand Genetic Position: Chr9, 71.37 cM
• Alliance: Cx3cr1^tm1Litt page

Retinal lesion in Ccl2^tm1Rol/Ccl2^tm1Rol Cx3cr1^tm1Litt/Cx3cr1^tm1Litt mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84544
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous locus was disrupted by the insertion of sequence encoding green fluourescent protein (GFP), replacing the first 390 bp of the coding exon (exon 2). The deleted region encoded an amino-terminal portion of the protein that is crucial for interaction with endogenous ligand, Cx3cl1. A floxed neo gene included in the targeting vector for selection was excised prior to germline transmission, leaving a single loxP site downstream of the GFP sequence. RT-PCR and flow cytometry indicated an absence of endogenous protein and the presence GFP expression in homozygous mutant mice. (J:84544)

Disease models

Expression

In Mice Carrying this Mutation:	190 assay results
In Structures Affected by this Mutation:	9 anatomical structures

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Cx3cr1 Mutation: 34 strains or lines available

References

• Original: J:84544 Jung S, et al., Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion. Mol Cell Biol. 2000 Jun;20(11):4106-14
• All: 880 reference(s)