Tg(Flt3-cre)#Ccb
Transgene Detail

Summary

• Symbol: Tg(Flt3-cre)#Ccb
• Name: transgene insertion, Conrad C Bleul
• MGI ID: MGI:4462354
• Synonyms: Flt3::Cre
• Transgene: Tg(Flt3-cre)#Ccb Location: unknown
• Alliance: Tg(Flt3-cre)#Ccb page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Flt3-cre)#Ccb expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Flt3 (MGI:95559)

• Mutation details: A sequence encoding cre together with an FRT-flanked kanamycin cassette was inserted into a BAC (RP24-145C8) into exon 1 of the Flt3 locus by ET cloning. Negative selection to identify properly targeted colonies was performed by transfection with an Flp recombinase-containing plasmid and selecting for kanamycin-sensitive colonies. Recombination was confirmed by sequencing and properly targeted BACs were injected into C57BL/6 pronuclei. Multiple lines were generated but no line numbers were specified. (J:136220)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Flt3 (mouse) Summary of all recombinase alleles driven by Flt3. Comparative matrix view of recombinase activities Alliance mouse Flt3 gene page

Disease models

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:136220 Benz C, et al., The stream of precursors that colonizes the thymus proceeds selectively through the early T lineage precursor stage of T cell development. J Exp Med. 2008 May 12;205(5):1187-99
• All: 48 reference(s)