Antibody:	anti-Aqp0 (AB3071)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS21: lens fiber	primary lens fiber	Present	Regionally restricted	6A	Expression is in the plasma-membranes of primary fiber cells that elongate from the posterior of the lens vesicle to fill the vesicle lumen.
TS21: posterior lens fiber	primary lens fiber	Present	Regionally restricted	6A	Expression is in the plasma-membranes of primary fiber cells that elongate from the posterior of the lens vesicle to fill the vesicle lumen.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: lens fiber	primary lens fiber	Present	Regionally restricted	6B	Expression is in the plasma-membranes of primary fiber cells that elongate from the posterior of the lens vesicle to fill the vesicle lumen.
TS21: posterior lens fiber	primary lens fiber	Present	Regionally restricted	6B	Expression is in the plasma-membranes of primary fiber cells that elongate from the posterior of the lens vesicle to fill the vesicle lumen.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: lens fiber	primary lens fiber	Present	Regionally restricted	6C	Expression in this mutant is similar to wild-type.
TS21: posterior lens fiber	primary lens fiber	Present	Regionally restricted	6C	Expression in this mutant is similar to wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: lens fiber	primary lens fiber	Present	Regionally restricted	6D	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.
TS23: lens fiber	secondary lens fiber	Present	Regionally restricted	6D	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.
TS23: posterior lens fiber	primary lens fiber	Present	Regionally restricted	6D	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.
TS23: posterior lens fiber	secondary lens fiber	Present	Regionally restricted	6D	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: lens fiber	primary lens fiber	Present	Regionally restricted	6E	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.
TS23: lens fiber	secondary lens fiber	Present	Regionally restricted	6E	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.
TS23: posterior lens fiber	primary lens fiber	Present	Regionally restricted	6E	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.
TS23: posterior lens fiber	secondary lens fiber	Present	Regionally restricted	6E	Expression is in the plasma-membranes of primary fiber cells and nascent secondary fiber cells.

Structure	Level	Pattern	Image	Note
TS23: lens	Present		6F	In this mutant expression is in random and grossly abnormal amorphous cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: lens fiber	lens fiber cell	Present	Regionally restricted	9A	Expression is in concentric tightly packed layers of fiber cell membranes.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: lens fiber	lens fiber cell	Present	Regionally restricted	9B	In this mutant fiber cell organization was variably disturbed with regions of large vesicle-like structures or fiber cell degeneration.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: lens fiber	lens fiber cell	Present	Regionally restricted	9C	In this mutant fiber cell organization was variably disturbed with regions of large vesicle-like structures or fiber cell degeneration.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: lens fiber	lens fiber cell	Present	Regionally restricted	9D	In this mutant fiber cell organization was variably disturbed with regions of large vesicle-like structures or fiber cell degeneration.