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Gene Expression Data
Assay Details
Assay
Reference: J:321982 De A, et al., The beta8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development. Development. 2022 Mar 15;149(6):dev200472
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281871
Gene symbol: Cspg4
Gene name: chondroitin sulfate proteoglycan 4
Antibody: anti-NG2 (AB5320)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S5G cortex: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Cspg4; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex brain pericyte Present S5G Cortex
Specimen S5G midbrain: embryonic day 18.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Cspg4; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: midbrain brain pericyte Present S5G Midbrain
Specimen S5H cortex: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Cspg4; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex brain pericyte Present S5H Cortex Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Specimen S5H midbrain: embryonic day 18.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Cspg4; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: midbrain brain pericyte Present S5H Midbrain Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Specimen S5I cortex: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Cspg4; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex brain pericyte Present S5I Cortex Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Specimen S5I midbrain: embryonic day 18.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Cspg4; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS26: midbrain brain pericyte Present S5I Midbrain Similar to controls, Pecam+ endothelial cells in mutant were associated with Cspg4+ pericytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory