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Gene Expression Data
Assay Details
Assay
Reference: J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6863898
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: anti-Nkx2.1 (mouse)
Detection system: Secondary antibody
Results
Specimen 1B - E12.5: embryonic day 12.5; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS20: lung epithelium Present Widespread 1B E12.5
Specimen 1B - E16.5: embryonic day 16.5; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS24: lung epithelium Present Widespread 1B E16.5
Specimen 1B - E18.5: embryonic day 18.5; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS26: lung epithelium Present Widespread 1B E18.5 , 1C Merge , 1C Nkx2.1 Many, but not all, expressing cells also express the NANCI reporter.
Specimen 1D - Bronchi: postnatal adult; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS28: lobar bronchus epithelium Present Widespread 1D Bronchi Merge , 1D Bronchi Nkx2.1 Many, but not all, expressing cells also express the NANCI reporter.
Specimen 1D - Small Airways: postnatal adult; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS28: bronchiole epithelium Present Widespread 1D Small Airways Merge , 1D Small Airways Nkx2.1 Many, but not all, expressing cells also express the NANCI reporter.
Specimen 1D - Alveoli: postnatal adult; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS28: alveolus epithelium Present Widespread 1D Alveoli Nkx2.1 , 1D Alveoli Merge Many, but not all, expressing cells also express the NANCI reporter.
Specimen S1B - E12.5: embryonic day 12.5; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS20: medial ganglionic eminence Present S1B E12.5 Merge , S1B E12.5 Nkx2.1
TS20: preoptic area Present S1B E12.5 Nkx2.1 , S1B E12.5 Merge Expression is seen in the anterior entopeduncular/preoptic area.
TS20: hypothalamus Present S1B E12.5 Merge , S1B E12.5 Nkx2.1
TS20: forebrain Present Regionally restricted S1B E12.5 Merge , S1B E12.5 Nkx2.1 Areas of expression include the developing globus pallidus.
Specimen S1B - Hypothalamus: embryonic day 18.5; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS26: hypothalamus Present S1B E18.5 Hypothalamus Nkx2.1 , S1B E18.5 Hypothalamus Merge Many, but not all, expressing cells also express the NANCI reporter.
Specimen S1B - Globus Pallidus: embryonic day 18.5; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Level Pattern Image Note
TS26: globus pallidus Present S1B E18.5 Globus Pallidus Nkx2.1 , S1B E18.5 Globus Pallidus Merge Many, but not all, expressing cells also express the NANCI reporter.
Specimen S2A: postnatal week 10; Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: green - Nkx2-1; red - Sfta3-ps reporter (assay MGI:6863897).
Structure Cell Type Level Pattern Image Note
TS28: thyroid gland epithelial cell of thyroid gland Present Regionally restricted S2A lower Merge , S2A upper Nkx2.1 , S2A lower Nkx2.1 , S2A upper Merge Expression is seen in the epithelium. Many, but not all, of these cells also express the NANCI reporter.
Specimen 1H - Sftpc: postnatal adult (more )
Note: Double labeled: red - Nkx2-1; green - Sftpc (assay MGI:6863924).
Structure Level Pattern Image Note
TS28: lung epithelium Present 1H top merge , 1H top Nkx2.1 Some expressing cells are pulmonary epithelium alveolar type 2 (AT2) cells.
Specimen 1H - Scgb1a1: postnatal adult (more )
Note: Double labeled: red - Nkx2-1; green - Scgb1a1 (assay MGI:6863929).
Structure Level Pattern Image Note
TS28: lung epithelium Present 1H center Nkx2.1 , 1H center merge Some expressing cells are secretory cells.
Specimen 1H - Foxj1: postnatal adult (more )
Note: Double labeled: red - Nkx2-1; green - Foxj1 (assay MGI:6863930).
Structure Level Pattern Image Note
TS28: lung epithelium Present 1H bottom Nkx2.1 , 1H bottom merge Some expressing cells are ciliated.
Specimen S3F - Pdpn: postnatal adult (more )
Note: Double labeled: red - Nkx2-1; green - Pdpn (assay MGI:6863931).
Structure Level Pattern Image Note
TS28: lung epithelium Present S3F top Nkx2.1 , S3F top merge Some expressing cells are pulmonary epithelium alveolar type 1 (AT1) cells.
Specimen S3F - Pgp9.5: postnatal adult (more )
Note: Double labeled: red - Nkx2-1; green - Uchl1 (assay MGI:6863932).
Structure Level Pattern Image Note
TS28: lung epithelium Present S3F 2nd down merge , S3F 2nd down Nkx2.1 Some expressing cells are neuroendocrine cells.
Specimen S3F - Muc5ac: postnatal adult (more )
Note: Double labeled: red - Nkx2-1; green - Muc5ac (assay MGI:6863934).
Structure Level Pattern Image Note
TS28: lobar bronchus epithelium Present S3F 3rd down Nkx2.1 , S3F 3rd down merge Expressing cells are seen in the mainstem bronchi; they are not goblet cells.
Specimen S3F - P63: postnatal adult (more )
Note: Double labeled: red - Nkx2-1; green - Trp63 (assay MGI:6863935).
Structure Level Pattern Image Note
TS28: lobar bronchus epithelium Present S3F bottom Nkx2.1 , S3F bottom merge Expressing cells are seen in the mainstem bronchi; some are basal cells.
Specimen 6F: postnatal month 6; Nkx2-1tm1Ikono/Nkx2-1+, Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+ (more )
Note: Double labeled: red - Nkx2-1; green - Trp63 (assay MGI:6863935).
Structure Level Pattern Image Note
TS28: lung epithelium Present 6F Expression is seen in the distal bronchiolized epithelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory