Muc5ac Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6863934
Gene symbol:	Muc5ac
Gene name:	mucin 5, subtypes A and C, tracheobronchial/gastric

Antibody:	anti-MUC5AC (45M1)
Detection system:	Secondary antibody

Results

Specimen S3E - Muc5ac: postnatal adult; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Muc5ac; red - Sfta3-ps reporter (assay MGI:6863897).

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus epithelium	Present	Single cells	S3E 3rd down Lineage Marker , S3E 3rd down merge	Expression is seen in goblet cells of the mainstem bronchi.

Specimen S3F - Muc5ac: postnatal adult (more

)
Note: Double labeled: green - Muc5ac; red - Nkx2-1 (assay MGI:6863898).

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus epithelium	Present	Single cells	S3F 3rd down Lineage Marker , S3F 3rd down merge	Expression is seen in goblet cells of the mainstem bronchi.

Specimen 5A - Wild-type: postnatal week 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A lower Wild-type

Specimen 5A - NANCI^{creERT2-RFP/+}: postnatal week 10; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A lower NANCI^RFP/+

Specimen 5A - Nkx2.1^GFP/+: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A lower Nkx2.1^GFP/+

Specimen 5A - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		5A lower NANCI^RFP/+; Nkx2.1^GFP/+	This expression is an indication of mucous cell metaplasia.

Specimen 6C - Wild-type: postnatal month 6 (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C bottom Wild-type

Specimen 6C - NANCI^{creERT2-RFP/+}: postnatal month 6; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C bottom NANCI^RFP/+

Specimen 6C - Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C bottom Nkx2.1^GFP/+

Specimen 6C - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	6C bottom NANCI^RFP/+; Nkx2.1^GFP/+	Goblet cell expression is expanded in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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