Trp63 Immunohistochemistry Gene Expression Assay - GXD

About Help FAQ

Gene Expression Data
Assay Details

Assay

Reference:	J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6863935
Gene symbol:	Trp63
Gene name:	transformation related protein 63

Antibody:	anti-P63
Detection system:	Secondary antibody

Results

S3E bottom Lineage Marker ©

S3E bottom merge ©

Specimen S3E - P63: postnatal adult; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Trp63; red - Sfta3-ps reporter (assay MGI:6863897).

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus epithelium	Present	Single cells	S3E bottom Lineage Marker , S3E bottom merge	Expression is seen in basal cells of the mainstem bronchi.

S3F bottom Lineage Marker ©

S3F bottom merge ©

Specimen S3F - P63: postnatal adult (more

)
Note: Double labeled: green - Trp63; red - Nkx2-1 (assay MGI:6863898).

Structure	Level	Pattern	Image	Note
TS28: lobar bronchus epithelium	Present	Single cells	S3F bottom merge , S3F bottom Lineage Marker	Expression is seen in basal cells of the mainstem bronchi.

5A upper Wild-type ©

Specimen 5A - Wild-type: postnatal week 10 (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A upper Wild-type

5A upper NANCI^RFP/+ ©

Specimen 5A - NANCI^{creERT2-RFP/+}: postnatal week 10; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A upper NANCI^RFP/+

5A upper Nkx2.1^GFP/+ ©

Specimen 5A - Nkx2.1^GFP/+: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		5A upper Nkx2.1^GFP/+

5A upper NANCI^RFP/+; Nkx2.1^GFP/+ ©

Specimen 5A - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		5A upper NANCI^RFP/+; Nkx2.1^GFP/+	This expression is an indication of basal cell metaplasia.

6C center Wild-type ©

Specimen 6C - Wild-type: postnatal month 6 (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C center Wild-type

6C center NANCI^RFP/+ ©

Specimen 6C - NANCI^{creERT2-RFP/+}: postnatal month 6; Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C center NANCI^RFP/+

6C center Nkx2.1^GFP/+ ©

Specimen 6C - Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Absent		6C center Nkx2.1^GFP/+

6C center NANCI^RFP/+; Nkx2.1^GFP/+ ©

Specimen 6C - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	6C center NANCI^RFP/+; Nkx2.1^GFP/+	Basal cell expression is expanded in the mutant.

6E ©

Specimen 6E: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Trp63; red - Krt5 (assay MGI:6863942).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	6E	Expression is seen in basal-like cells in the distal bronchiolized epithelium.

6F ©

Specimen 6F: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3-ps^{tm1.1(cre/ERT2)Eem}/Sfta3-ps⁺ (more

)
Note: Double labeled: green - Trp63; red - Nkx2-1 (assay MGI:6863898).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	6F	Expression is seen in basal-like cells in the distal bronchiolized epithelium.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/07/2024 MGI 6.23