Gene Expression Data
Assay Details
Assay
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Reference:
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J:243650
Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6863924
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Gene symbol:
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Sftpc
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Gene name:
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surfactant associated protein C
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Antibody:
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anti-Sftpc
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Detection system:
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Secondary antibody
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Results
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Specimen
1F - Sftpc:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Sfta3-ps reporter (assay MGI:6863897).
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Specimen
1H - Sftpc:
postnatal adult
(more )
Note: Double labeled: green - Sftpc; red - Nkx2-1 (assay MGI:6863898).
Specimen
1H - Sftpc:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Nkx2-1 (assay MGI:6863898).
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Specimen
3B - Wild-type:
embryonic day 18.5
(more )
Specimen
3B - Wild-type:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B - NANCI:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
Present |
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3B lower NANCIRFP/+
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Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are comparable to those seen in wild type.
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Specimen
3B - Nkx2.1GFP/+:
embryonic day 18.5; Nkx2-1tm1Ikono/Nkx2-1 +
(more )
Specimen
3B - Nkx2.1:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx2-1tm1Ikono/Nkx2-1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
Present |
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3B lower Nkx2.1GFP/+
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Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are significantly reduced compared to those seen in wild type.
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Specimen
3B - NANCI; Nkx2.1:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx2-1tm1Ikono/Nkx2-1+ Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3E - Wild-type:
postnatal day 4
(more )
Specimen
3E - Wild-type:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3E - NANCI:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: lung epithelium |
Present |
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3E lower NANCIRFP/+
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Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are reduced compared to those seen in wild type.
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Specimen
3E - Nkx2.1GFP/+:
postnatal day 4; Nkx2-1tm1Ikono/Nkx2-1 +
(more )
Specimen
3E - Nkx2.1:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx2-1tm1Ikono/Nkx2-1+
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lung epithelium |
Present |
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3E lower Nkx2.1GFP/+
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Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are reduced compared to those seen in wild type.
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Specimen
3E - NANCI; Nkx2.1:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx2-1tm1Ikono/Nkx2-1+ Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5E:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx2-1tm1Ikono/Nkx2-1+ Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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postnatal week 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Sox2 (assay MGI:6863946).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: lung epithelium |
Present |
Single cells |
5E
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Expression is seen in a small subset of the bronchiolized cells in the distal lung.
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Specimen
5F:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx2-1tm1Ikono/Nkx2-1+ Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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postnatal week 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Sftpc; green - Scgb1a1 (assay MGI:6863929).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: lung epithelium |
Present |
Single cells |
5F
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Expression is seen in a small subset of the bronchiolized cells in the distal lung.
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Specimen
S8J:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Nkx2-1tm1Ikono/Nkx2-1+ Sfta3-pstm1.1(cre/ERT2)Eem/Sfta3-ps+
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sftpc; red - Mki67 (assay MGI:6863973).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: lung |
Present |
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S8J
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Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; they are not showing an increase in proliferation in the mutant. This is quantified in Fig. S8K.
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