About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6758952
Gene symbol: Lyve1
Gene name: lymphatic vessel endothelial hyaluronan receptor 1
Antibody: anti-LYVE1 (AF2125)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1A: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1A LYVE1 , 1A merge Expressed extensively in cardiac lymphatic vessels.
TS26: lymphatic vessel endothelium Present Regionally restricted 1A LYVE1 , 1A merge Expressed extensively in cardiac lymphatic vessels.
Specimen 1B: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1B LYVE1 , 1B merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
TS26: lymphatic vessel endothelium Present Regionally restricted 1B LYVE1 , 1B merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen 1C: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present 1C LYVE1 , 1C merge Expressed in cardiac lymphatic vessels.
TS26: lymphatic vessel endothelium Present 1C LYVE1 , 1C merge Expressed in cardiac lymphatic vessels.
Specimen 1D: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1D LYVE1 , 1D merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
TS26: lymphatic vessel endothelium Present Regionally restricted 1D LYVE1 , 1D merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen 1E: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1E merge , 1E LYVE1 Expressed extensively in cardiac lymphatic vessels.
TS26: lymphatic vessel endothelium Present Regionally restricted 1E merge , 1E LYVE1 Expressed extensively in cardiac lymphatic vessels.
Specimen 1F: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1F merge , 1F LYVE1 Expressed in hypoplastic cardiac lymphatic vessels. Scarce lymphatic vessels on the dorsal ventricular wall.
TS26: lymphatic vessel endothelium Present Regionally restricted 1F merge , 1F LYVE1 Expressed in hypoplastic cardiac lymphatic vessels. Scarce lymphatic vessels on the dorsal ventricular wall.
Specimen 3A/B: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3B , 3A Expressed in cardiac lymphatic vessels.
Specimen 3C/D: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3D , 3C Expressed in cardiac lymphatic vessels.
Specimen 3E/F: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3E , 3F Decreased expression in impaired lymphatic vessels particularly on the ventral side.
Specimen 3G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3H , 3G Decreased expression in impaired lymphatic vessels particularly on the ventral side.
Specimen 3I/J: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3J LYVE1 , 3I , 3J Merge Expressed in lymphatic vessels around the aorta on the ventral side of the heart.
Specimen 3K/L: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3K , 3L LYVE1 , 3L Merge Decreased expression in impaired lymphatic vessels and did not apparently form mature thick vessels.
Specimen 6A: embryonic day 12.0 (more )
Note: Left lateral. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 6A LYVE1 , 6A Prox1-LYVE1 , 6A Merge Expressed in vessels around the sinus venosus. No coexpression with Prox1 mostly.
Specimen S1D fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels.
TS25: lymphatic vessel endothelium Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels.
Specimen S1D fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1D right Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
TS25: lymphatic vessel endothelium Present Regionally restricted S1D right Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen S1E/F fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1F LYVE1 left , S1E left , S1F merge left Expressed in cardiac lymphatic vessels.
TS25: lymphatic vessel endothelium Present Regionally restricted S1F LYVE1 left , S1E left , S1F merge left Expressed in cardiac lymphatic vessels.
Specimen S1E/F fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1E right , S1F merge right , S1F LYVE1 right Expressed in hypoplastic cardiac lymphatic vessels. Aberrant Lyve1-negative microvasculature was formed by Foxa1+/Pecam1+ cells around the aortic stem sites.
TS25: lymphatic vessel endothelium Present Regionally restricted S1E right , S1F merge right , S1F LYVE1 right Expressed in hypoplastic cardiac lymphatic vessels. Aberrant Lyve1-negative microvasculature was formed by Foxa1+/Pecam1+ cells around the aortic stem sites.
Specimen S3A/B/C: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; blue - Prox1 (assay MGI:6758992); green - Cre reporter.
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted S3C LYVE1 , S3A , S3C merge , S3B Colocalized with Isl1+ lineage and Prox1 around the outflow tract.
Specimen S3D/E: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Prox1 (assay MGI:6758992); green - Cre reporter.
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted S3D , S3E merge , S3E LYVE1 Colocalized with Prox1, not the Isl1+ lineage on the dorsal side of the heart.
Specimen S5A/B: embryonic day 14.5 (more )
Note: Ventral side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS22: arch of aorta Present Scattered S5B merge , S5A , S5B LYVE1 Sparsely coexpressed with Adgre1 (likely macrophages) on the aortic wall.
TS22: lymphatic vessel endothelium Present S5B merge , S5A , S5B LYVE1
Specimen S5C: embryonic day 14.5 (more )
Note: Dorsal side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS22: heart Present Scattered S5C merge , S5C LYVE1 A small number of Adgre1+/Lyve1+ macrophages were identified around the sinus venosus.
TS22: lymphatic vessel endothelium Present S5C merge , S5C LYVE1
Specimen S5D/E: embryonic day 14.5 (more )
Note: Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS22: visceral pericardium Present Scattered S5E LYVE1 , S5D , S5E merge Sparsely coexpressed with Lyve1 in macrophages.
TS22: lymphatic vessel endothelium Present S5E LYVE1 , S5E merge , S5D
Specimen S5F/G: embryonic day 15.5 (more )
Note: Ventral side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS23: aorta Present Scattered S5F , S5G merge , S5G LYVE1 Coexpressed with Adgre1 in macrophages.
TS23: lymphatic vessel endothelium Present S5G merge , S5G LYVE1 , S5F
Specimen S5H: embryonic day 15.5 (more )
Note: Dorsal side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS23: visceral pericardium Present Scattered S5H LYVE1 , S5H merge Coexpressed with Adgre1 in macrophages.
TS23: lymphatic vessel endothelium Present S5H LYVE1 , S5H merge
Specimen S5I/J: embryonic day 15.5 (more )
Note: Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS23: visceral pericardium Present Scattered S5I , S5J merge , S5J LYVE1 Coexpressed with Adgre1 in macrophages.
TS23: lymphatic vessel endothelium Present S5J merge , S5J LYVE1 , S5I
Specimen S5K/L: embryonic day 16.5 (more )
Note: Ventral side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS24: aorta Present Scattered S5L LYVE1 , S5L merge , S5K Coexpressed with Adgre1 in macrophages.
TS24: lymphatic vessel endothelium Present S5L merge , S5K , S5L LYVE1
Specimen S5M: embryonic day 16.5 (more )
Note: Dorsal side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS24: visceral pericardium Present Scattered S5M merge , S5M LYVE1 Coexpressed with Adgre1 in macrophages.
TS24: lymphatic vessel endothelium Present S5M LYVE1 , S5M merge
Specimen S5N/O: embryonic day 16.5 (more )
Note: Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS24: visceral pericardium Present Scattered S5O merge , S5N , S5O LYVE1 Coexpressed with Adgre1 in macrophages.
TS24: lymphatic vessel endothelium Present S5N , S5O merge , S5O LYVE1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory