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Gene Expression Data
Assay Details
Assay
Reference: J:308866 Mase S, et al., Notch1 and Notch2 collaboratively maintain radial glial cells in mouse neurogenesis. Neurosci Res. 2021 Sep;170:122-132
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6755498
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: anti-Sox2 (clone Btjce)
Detection system: Alexa Fluor 488
Results
Specimen 1A Notch1+/D Notch2+/D: embryonic day 11.5; Emx1tm1(cre)Yql/Emx1+, Notch1tm2Rko/Notch1+, Notch2tm3Grid/Notch2+ (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS19: telencephalon Present 1A Notch1+/D Notch2+/D Sox2 , 1A Notch1+/D Notch2+/D merge Expression in neural stem cells (NSCs) in dorsal cortex.
Specimen 1A Notch1D/D: embryonic day 11.5; Emx1tm1(cre)Yql/Emx1+, Notch1tm2Rko/Notch1tm2Rko (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS19: telencephalon Present 1A Notch1D/D merge , 1A Notch1D/D Sox2 Elimination of Notch1 greatly reduced radial glial cells (RGC) cell number/unit area in dorsal cortex.
Specimen 1A Notch2D/D: embryonic day 11.5; Emx1tm1(cre)Yql/Emx1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS19: telencephalon Present 1A Notch2D/D Sox2 , 1A Notch2D/D merge No significant change in radial gial cell (RGC) cell number/unit area.
Specimen 1A Notch1D/D Notch2D/D: embryonic day 11.5; Emx1tm1(cre)Yql/Emx1+, Notch1tm2Rko/Notch1tm2Rko, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS19: telencephalon Present 1A Notch1D/D Notch2D/D Sox2 , 1A Notch1D/D Notch2D/D merge Elimination of Notch1 greatly reduced radial glial cells (RGC) cell number/unit area in dorsal cortex.
Specimen 1B Notch1+/D Notch2+/D: embryonic day 13.5; Emx1tm1(cre)Yql/Emx1+, Notch1tm2Rko/Notch1+, Notch2tm3Grid/Notch2+ (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS21: cerebral cortex Present 1B Notch1+/D Notch2+/D Sox2 , 1B Notch1+/D Notch2+/D merge Expression in neural stem cells (NSCs) in dorsal cortex.
Specimen 1B Notch1D/D: embryonic day 13.5; Emx1tm1(cre)Yql/Emx1+, Notch1tm2Rko/Notch1tm2Rko (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS21: cerebral cortex Present 1B Notch1D/D Sox2 , 1B Notch1D/D merge Elimination of Notch1 greatly reduced radial glial cells (RGC) cell number/unit area in dorsal cortex.
Specimen 1B Notch2D/D: embryonic day 13.5; Emx1tm1(cre)Yql/Emx1+, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS21: cerebral cortex Present 1B Notch2D/D merge , 1B Notch2D/D Sox2 No significant change in radial gial cell (RGC) cell number/unit area.
Specimen 1B Notch1D/D Notch2D/D: embryonic day 13.5; Emx1tm1(cre)Yql/Emx1+, Notch1tm2Rko/Notch1tm2Rko, Notch2tm3Grid/Notch2tm3Grid (more )
Note: Conditional mutant. Triple labeled: green - Sox2; magenta - Tbr2 (Eomes assay MGI:6755499); blue - DAPI.
Structure Level Pattern Image Note
TS21: cerebral cortex Present 1B Notch1D/D Notch2D/D Sox2 , 1B Notch1D/D Notch2D/D merge Only a small number of proliferating radial glial cells (RGCs) remained, and epithelial cells in dorsal cortex was compromised.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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