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Gene Expression Data
Assay Details
Assay
Reference: J:256775 Zhang H, et al., Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Circ Res. 2016 Jun 10;118(12):1880-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6723846
Gene symbol: Cdh5
Gene name: cadherin 5
Antibody: anti-VE-cadherin (AF1002)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen Online 1C: embryonic day 13.5; Nfatc1tm2(cre)Bzsh/Nfatc1+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: gray - Cdh5; green - Fabp4 (assay MGI:6723819); red - Cre reporter.
Structure Level Pattern Image Note
TS21: heart endothelium Present Online 1C1 , Online 1C2 , Online 1C merge , Online 1C gray Expression is seen in endothelial cells.
TS21: ventricle endocardial lining Present Online 1C1 , Online 1C2 , Online 1C merge , Online 1C gray Expression is seen in endothelial cells.
Specimen 4A - right: embryonic day 9.5; Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Nfatc1em3(dre)Bzsh/Nfatc1+, Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Conditional mutant. Double labeled: gray - Cdh5; green - Npr3 reporter (assay MGI:6724008).
Structure Level Pattern Image Note
TS15: primitive ventricle endocardial lining Present 4A green/gray merge , 4A green/gray merge inset Expression is seen in endothelial cells.
TS15: atrium endocardial lining Present 4A green/gray merge Expression is seen in endothelial cells.
TS15: sinus venosus Present Regionally restricted 4A green/gray merge Expression is seen in endothelial cells.
Specimen 4B - right: embryonic day 10.5; Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Nfatc1em3(dre)Bzsh/Nfatc1+, Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Conditional mutant. Double labeled: gray - Cdh5; green - Npr3 reporter (assay MGI:6724008).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 4B green/gray merge , 4B green/gray merge inset Expression is seen in endothelial cells.
TS17: atrium endocardial lining Present 4B green/gray merge Expression is seen in endothelial cells.
TS17: sinus venosus Present Regionally restricted 4B green/gray merge Expression is seen in endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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