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Gene Expression Data
Assay Details
Assay
Reference: J:256775 Zhang H, et al., Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Circ Res. 2016 Jun 10;118(12):1880-93
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6724008
Gene symbol: Npr3
Gene name: natriuretic peptide receptor 3
Reporter: ESR
Antibody: anti-ESR (ab27595)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4A - left: embryonic day 9.5; Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Nfatc1em3(dre)Bzsh/Nfatc1+, Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Conditional mutant. Double labeled: green - Npr3 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS15: primitive ventricle endocardial lining Present 4A green/red merge , 4A green , 4A green/red merge inset , 4A green inset
TS15: atrium endocardial lining Present 4A green , 4A green/red merge
TS15: sinus venosus Absent 4A green/red merge , 4A green
Specimen 4A - right: embryonic day 9.5; Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Nfatc1em3(dre)Bzsh/Nfatc1+, Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Conditional mutant. Double labeled: green - Npr3 reporter; gray - Cdh5 (assay MGI:6723846).
Structure Level Pattern Image Note
TS15: primitive ventricle endocardial lining Present 4A green/gray merge , 4A green/gray merge inset
TS15: atrium endocardial lining Present 4A green/gray merge
TS15: sinus venosus Absent 4A green/gray merge
Specimen 4B - left: embryonic day 10.5; Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Nfatc1em3(dre)Bzsh/Nfatc1+, Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Conditional mutant. Double labeled: green - Npr3 reporter; red - Cre reporter.
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 4B green inset , 4B green/red merge inset , 4B green/red merge , 4B green
TS17: atrium endocardial lining Present 4B green , 4B green/red merge
TS17: sinus venosus Absent 4B green/red merge , 4B green
Specimen 4B - right: embryonic day 10.5; Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Nfatc1em3(dre)Bzsh/Nfatc1+, Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Conditional mutant. Double labeled: green - Npr3 reporter; gray - Cdh5 (assay MGI:6723846).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 4B green/gray merge inset , 4B green/gray merge
TS17: atrium endocardial lining Present 4B green/gray merge
TS17: sinus venosus Absent 4B green/gray merge
Specimen Online 3A - upper: embryonic day 8.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Cdh5 (assay MGI:6723847).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3A upper merge , Online 3A upper inset , Online 3A upper green
TS13: common atrial chamber endocardial tube Present Online 3A upper green , Online 3A upper merge
Specimen Online 3A - center: embryonic day 8.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Cdh5 (assay MGI:6723847).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3A center green , Online 3A center merge , Online 3A center inset
TS13: sinus venosus Absent Online 3A center green , Online 3A center inset , Online 3A center merge
Specimen Online 3A - lower: embryonic day 8.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Cdh5 (assay MGI:6723847).
Structure Level Pattern Image Note
TS13: sinus venosus Absent Online 3A lower green , Online 3A lower inset , Online 3A lower merge
Specimen Online 4A: embryonic day 9.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Tbx18 (assay MGI:6724012).
Structure Level Pattern Image Note
TS15: primitive ventricle endocardial lining Present Online 4A green inset , Online 4A merge inset , Online 4A merge , Online 4A green
TS15: common atrial chamber endocardial lining Present Online 4A merge , Online 4A green
TS15: proepicardium Absent Online 4A green , Online 4A green inset , Online 4A merge inset , Online 4A merge
Specimen Online 4B: embryonic day 10.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: green - Npr3 reporter; red - Tbx18 (assay MGI:6724012).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present Online 4B green , Online 4B merge , Online 4B merge inset , Online 4B green inset
TS17: visceral pericardium Weak Single cells Online 4B merge inset , Online 4B green inset , Online 4B green , Online 4B merge Expression is seen in a few epicardial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory