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Gene Expression Data
Assay Details
Assay
Reference: J:256775 Zhang H, et al., Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Circ Res. 2016 Jun 10;118(12):1880-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6723847
Gene symbol: Cdh5
Gene name: cadherin 5
Antibody: anti-VE-cadherin (AF1002)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen Online 2C: embryonic day 15.5; Nfatc1em2(GFP/cre)Bzsh/Nfatc1+ (more )
Note: Double labeled: red - Cdh5; green - Nfatc1 reporter (assay MGI:6721137).
Structure Level Pattern Image Note
TS23: heart endothelium Present Online 2C right center , Online 2C right upper , Online 2C right lower

Specimen Online 2D: embryonic day 15.5 (more )
Note: Double labeled: red - Cdh5; green - Nfatc1 protein (assay MGI:6723858).
Structure Level Pattern Image Note
TS23: heart endothelium Present Online 2D merge inset , Online 2D merge , Online 2D red , Online 2D red inset

Specimen 3D: embryonic day 10.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: red - Cdh5; green - Npr3 reporter (assay MGI:6723865).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 3D red , 3D1 red , 3D merge , 3D1 merge Expression is seen in endothelial cells.
TS17: atrium endocardial lining Present 3D red , 3D merge , 3D2 red , 3D2 merge Expression is seen in endothelial cells.
TS17: sinus venosus Present Regionally restricted 3D2 red , 3D2 merge , 3D red , 3D merge Expression is seen in endothelial cells.

Specimen Online 3A - upper: embryonic day 8.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: red - Cdh5; green - Npr3 reporter (assay MGI:6724008).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3A upper red , Online 3A upper merge , Online 3A upper inset
TS13: common atrial chamber endocardial tube Present Online 3A upper red , Online 3A upper merge

Specimen Online 3A - center: embryonic day 8.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: red - Cdh5; green - Npr3 reporter (assay MGI:6724008).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3A center red , Online 3A center merge , Online 3A center inset
TS13: sinus venosus Present Online 3A center red , Online 3A center merge , Online 3A center inset

Specimen Online 3A - lower: embryonic day 8.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: red - Cdh5; green - Npr3 reporter (assay MGI:6724008).
Structure Level Pattern Image Note
TS13: sinus venosus Present Online 3A lower inset , Online 3A lower merge , Online 3A lower red

Specimen Online 3B - upper: embryonic day 8.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: red - Cdh5; green - Npr3 reporter (assay MGI:6723865).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3B upper red , Online 3B upper inset , Online 3B upper merge

Specimen Online 3B - center: embryonic day 8.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: red - Cdh5; green - Npr3 reporter (assay MGI:6723865).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3B center inset , Online 3B center merge , Online 3B center red
TS13: common atrial chamber endocardial tube Present Online 3B center merge , Online 3B center red

Specimen Online 3B - lower: embryonic day 8.5; Npr3tm2(cre/GFP)Bzsh/Npr3+ (more )
Note: Double labeled: red - Cdh5; green - Npr3 reporter (assay MGI:6723865).
Structure Level Pattern Image Note
TS13: primitive ventricle endocardial tube Present Online 3B lower merge , Online 3B lower inset , Online 3B lower red
TS13: sinus venosus Present Online 3B lower merge , Online 3B lower inset , Online 3B lower red


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory