About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:135698 Burlison JS, et al., Pdx-1 and Ptf1a concurrently determine fate specification of pancreatic multipotent progenitor cells. Dev Biol. 2008 Apr 1;316(1):74-86
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6695715
Gene symbol: Ptf1a
Gene name: pancreas specific transcription factor, 1a
Reporter: YFP
Antibody: anti-GFP
Results
Specimen 2 E10.5 YFP/+ (upper): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS17: neural tube Present Uniform 2_E10.5 YFP/+ (upper)
Specimen 2 E10.5 YFP/+ (lower): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Uniform 2_E10.5 YFP/+ (lower)
TS17: pancreas primordium ventral bud Present Uniform 2_E10.5 YFP/+ (lower)
Specimen 2 E10.5 YFP/YFP (upper): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS17: neural tube Present 2_E10.5 YFP/YFP (upper)
Specimen 2 E10.5 YFP/YFP (lower): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 2_E10.5 YFP/YFP (lower) Expression initiated in dorsal and ventral pancreatic buds at E10.5.
TS17: pancreas primordium ventral bud Present 2_E10.5 YFP/YFP (lower) Expression initiated in dorsal and ventral pancreatic buds at E10.5.
Specimen 2 E11.5 YFP/+ (upper): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Strong 2_E11.5 YFP/+ (upper)
TS19: pancreas primordium ventral bud Strong 2_E11.5 YFP/+ (upper)
Specimen 2 E11.5 YFP/+ (lower): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Strong 2_E11.5 YFP/+ (lower) strong / NS
TS19: pancreas primordium ventral bud Strong 2_E11.5 YFP/+ (lower) strong / NS
Specimen 2 E11.5 YFP/YFP (upper): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present 2_E11.5 YFP/YFP (upper)
Specimen 2 E11.5 YFP/YFP (lower): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present 2_E11.5 YFP/YFP (lower) Expression in both dorsal and ventral pancreatic buds at E11.5
TS19: pancreas primordium ventral bud Present 2_E11.5 YFP/YFP (lower) Expression in both dorsal and ventral pancreatic buds at E11.5
Specimen 2 E12.5 YFP/+: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS20: pancreas epithelium Strong Widespread 2_E12.5 YFP/+ Expression throughout pancreatic epithelium upon fusion of dorsal and ventral pancreas at E12.5.
Specimen 2 E12.5 YFP/YFP: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 2_E12.5 YFP/YFP Expression in ventral pancreatic remnant disappears by E12.5.
Specimen 2 E13.5 YFP/+ (upper): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS21: pancreatic acinus Strong Punctate 2_E13.5 YFP/+ (upper) At E13.5 expression is punctate, appearing as many clusters, denoting acinar restriction.
Specimen 2 E13.5 YFP/+ (lower): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS21: pancreas Strong Punctate 2_E13.5 YFP/+ (lower) At E13.5 expression is punctate, appearing as many clusters, denoting acinar restriction.
TS21: pancreatic acinus Strong Punctate 2_E13.5 YFP/+ (lower) At E13.5 expression is punctate, appearing as many clusters, denoting acinar restriction.
Specimen 2 E13.5 YFP/YFP (upper): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 2_E13.5 YFP/YFP (upper) Expression in dorsal pancreatic remnant persists until E13.5.
Specimen 2 E13.5 YFP/YFP (lower): embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 2_E13.5 YFP/YFP (lower) Expression in dorsal pancreatic remnant persists until E13.5.
Specimen 2 E15.5 YFP/+: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS23: pancreatic acinus Very strong Regionally restricted 2_E15.5 YFP/+ Intensity of expression increased further, corresponding with expansion of exocrine cells.
Specimen 2 E15.5 YFP/YFP: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS23: pancreas Absent 2_E15.5 YFP/YFP Expression in dorsal pancreatic remnant no longer seen at E15.5.
Specimen adult not shown: postnatal adult; Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS28: pancreas Strong Widespread Authors report pancreas-wide expression similar to that observed in late embryogenesis.
Specimen 3I: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 3I Expression only in dorsal pancreatic remnant from E10.5 to E12.5.
Specimen 3J: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 3J Expression only in dorsal pancreatic remnant from E10.5 to E12.5.
Specimen 3K: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 3K Expression only in dorsal pancreatic remnant from E10.5 to E12.5.
Specimen 3L: embryonic day 12.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 3L Expression only in dorsal pancreatic remnant from E10.5 to E12.5.
Specimen E13.5 LacZko/LacZko;YFP/+ not shown: embryonic day 13.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1a+ (more )
Structure Level Pattern Image Note
TS21: pancreas Absent
Specimen E13.5 LacZko/LacZko;YFP/YFP not shown: embryonic day 13.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Structure Level Pattern Image Note
TS21: pancreas Absent
Specimen 5A YFP (upper): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted 5A YFP (upper) , 5A Merge (upper) Expression coincident with Pdx1 throughout dorsal pancreatic epithelium, except for first wave endocrine cells.
TS17: duodenum Absent 5A YFP (upper) , 5A Merge (upper)
Specimen 5A YFP (lower): embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Gcg (assay MGI:6695729).
Structure Level Pattern Image Note
TS17: pancreas primordium ventral bud Present Regionally restricted 5A YFP (lower) , 5A Merge (lower) Expression coincident with Pdx1 throughout ventral pancreatic epithelium, except for first wave endocrine cells.
TS17: duodenum Absent 5A Merge (lower) , 5A YFP (lower)
TS17: extrahepatic bile duct Absent 5A Merge (lower) , 5A YFP (lower) No expression in common bile duct.
Specimen 5C E13.5: embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS21: pancreatic acinus Present 5C E13.5 Expression restricted to pro-acinar structures.
Specimen 5C E15.5: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS23: pancreatic acinus Present 5C E15.5 Expression restricted to pro-acinar structures.
Specimen 5D (middle): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Neurog3 (assay MGI:6695730).
Structure Level Pattern Image Note
TS19: pancreas primordium dorsal bud Present Widespread 5D (middle) Expression thoughout dorsal pancreatic remnant.
Specimen 5D (right): embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Double labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727).
Structure Level Pattern Image Note
TS19: pancreas primordium ventral bud Present 5D (right)
Specimen S1 YFP E9.5: embryonic day 9.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Present Widespread S1_E9.5 YFP , S1_E9.5 Merge Expression throughout dorsal and ventral pancreatic buds.
TS15: pancreas primordium ventral bud Present Widespread S1_E9.5 YFP , S1_E9.5 Merge Expression throughout dorsal and ventral pancreatic buds.
Specimen S1 YFP E10.5: embryonic day 10.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1_E10.5 YFP , S1_E10.5 Merge
Specimen S1 YFP E11.5: embryonic day 11.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS19: pancreas primordium Present S1_E11.5 YFP , S1_E11.5 Merge
Specimen S1 YFP E12.5: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS20: pancreas Strong Regionally restricted S1_E12.5 Merge , S1_E12.5 YFP Intense expression co-localizing with that of Ptf1a, except for some weaker expression towards central part of pancreas epithelium.
Specimen S1 YFP E13.5: embryonic day 13.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS21: pancreatic acinus Present S1_E13.5 YFP , S1_E13.5 Merge From E13.5 on, expression coincident with Ptf1a in all acinar cells.
Specimen S1 YFP E15.5: embryonic day 15.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Double labeled: green - Ptf1a reporter; red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS23: pancreatic acinus Present S1_E15.5 YFP , S1_E15.5 Merge From E13.5 on, expression coincident with Ptf1a in all acinar cells.
Specimen S2A YFP: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1a+ (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted S2A Merge , S2A YFP Expression in dorsal pancreas.
Specimen S2B YFP: embryonic day 12.5; Ptf1atm2.1Mgn/Ptf1atm2.1Mgn (more )
Note: Triple labeled: green - Ptf1a reporter; blue - Pdx1 (assay MGI:6695727); red - Ptf1a protein (assay MGI:6695726).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted S2B Merge , S2B YFP Expression in dorsal pancreatic remnant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory