About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:193583 Cai Y, et al., Nuclear receptor COUP-TFII-expressing neocortical interneurons are derived from the medial and lateral/caudal ganglionic eminence and define specific subsets of mature interneurons. J Comp Neurol. 2013 Feb 1;521(2):479-97
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6490533
Gene symbol: Nr2f2
Gene name: nuclear receptor subfamily 2, group F, member 2
Antibody: anti-COUP-TFII (PP-H7147-00)
Detection system: Secondary antibody
Results
Specimen 2A/B: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
Structure Level Pattern Image Note
TS24: lateral ganglionic eminence Present Regionally restricted 2A , 2B Expressed in the dorsal region, mainly in the subventricular zone with increasing expression from anterior to posterior.
TS24: cerebral cortex subventricular zone Present 2A , 2B
TS24: neocortex Present Regionally restricted 2A , 2B There was a migratory stream from the dorsal lateral ganglionic eminence to the neocortex through the subventricular zone. The majority of cells in the stream were Nr2f2+Sp8+.

Specimen 2E/F: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
Structure Level Pattern Image Note
TS24: lateral ganglionic eminence Present Regionally restricted 2F , 2E Expressed in the dorsal region, mainly in the subventricular zone with increasing expression from anterior to posterior.
TS24: medial ganglionic eminence Present Regionally restricted 2F , 2E Expressed in the dorsal region, mainly in the subventricular zone with increasing expression from anterior to posterior.
TS24: neocortex Present Regionally restricted 2F , 2E There was a migratory stream from the dorsal lateral ganglionic eminence to the neocortex through the subventricular zone. The majority of cells in the stream were Nr2f2+Sp8+.
TS24: cerebral cortex subventricular zone Present 2F , 2E

Specimen 2I/J: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
Structure Level Pattern Image Note
TS24: amygdala Present 2I , 2J
TS24: caudal ganglionic eminence Present 2I , 2J
TS24: cerebral cortex subventricular zone Present 2I , 2J
TS24: neocortex Present Regionally restricted 2I , 2J There was a migratory stream from the dorsal caudal ganglionic eminence to the neocortex through the subventricular zone. The majority of cells in the stream were Nr2f2+Sp8+.

Specimen 4A/B/C/D/E: postnatal day 28 (more )
Note: Double labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541).
Structure Level Pattern Image Note
TS28: cortical layer V Present 4D , 4E , 4A , 4C , 4B Nr2r2+Sst+ interneurons were mainly in layer V of the motor and somatosensory cortex.
TS28: cortical layer IV Present 4A A very small number of Nr2r2+Sst+ interneurons were found.
TS28: cortical layer VI Present 4A A very small number of Nr2r2+Sst+ interneurons were found.

Specimen 4F/G: postnatal day 28 (more )
Note: Double labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541).
Structure Level Pattern Image Note
TS28: cortical layer V Present 4G , 4F Nr2r2+Sst+ interneurons were mainly in layer V of the visual cortex.
TS28: cortical layer IV Present 4G A very small number of Nr2r2+Sst+ interneurons were found.
TS28: cortical layer VI Present 4G A very small number of Nr2r2+Sst+ interneurons were found.

Specimen 4H/I: postnatal day 28 (more )
Note: Triple labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541); blue - Calb2 (assay MGI:6490544).
Structure Level Pattern Image Note
TS28: cortical layer V Present 4I , 4H Nr2r2+Sst+ interneurons were mainly in layer V. No Nr2r2+Sst+Calb2+ neocortical interneurons.

Specimen 5A/B: postnatal day 0 (more )
Note: Triple labeled: magenta - Nr2f2; blue - Sp8 (assay MGI:6490539); green - Sox6 (assay MGI:6490545).
Structure Level Pattern Image Note
TS27: cortical layer V Present 5A , 5B Majority of Nr2f2+Sox6+ cells localized in layer V.

Specimen 6A/C: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E12.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present 6C , 6A The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5.

Specimen 6B/D: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E14.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer I Present 6D , 6B The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5.
TS28: cortical layer II Present 6D , 6B The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5.
TS28: cortical layer III Present 6D , 6B The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5.

Specimen 7E: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Shhtm2(cre/ERT2)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: magenta - Nr2f2; green - green - Cre reporter (Shh).
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted 7E Not expressed in the cell stream migrating from the preoptic area and ventral medial ganglionic eminence into the lateral ganglionic eminence.

Specimen 7J/L: postnatal adult; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Shhtm2(cre/ERT2)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: magenta - Nr2f2; green - green - Cre reporter (Shh).
Structure Level Pattern Image Note
TS28: neocortex Present 7L , 7J No expression in the pyramidal neurons migrated from the preoptic area and the medial ganglionic eminence (Shh-lineage).

Specimen 8A/B/E/F: postnatal adult; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Triple labeled: magenta - Nr2f2; blue - Sst (assay MGI:6490542); green - Cre reporter (Nkx2-1).
Structure Level Pattern Image Note
TS28: cortical layer V Present 8B , 8F , 8E , 8A Expressed in the Nkx2-1-lineage cells.

Specimen not shown: embryonic day 13.5 (more )
Note: Double labeled with histone H3.
Structure Level Pattern Image Note
TS21: medial ganglionic eminence Present Regionally restricted Nr2f2+pH3+ cells were seen in the ventricular zone of the dorsal region.

Specimen not shown: embryonic day 16.5 (more )
Note: Double labeled with histone H3.
Structure Level Pattern Image Note
TS24: lateral ganglionic eminence Present Regionally restricted No Nr2f2+pH3+ cells were seen in the subventricular zone of the dorsal region.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E10.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E11.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E13.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E15.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E16.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E17.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E18.5 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.

Specimen not shown: postnatal day 28 (more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at P0 and sacrificed at P28.
Structure Level Pattern Image Note
TS28: cortical layer V Present The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
TS28: cortical layer I Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer II Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
TS28: cortical layer III Present The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
05/07/2024
MGI 6.23
The Jackson Laboratory