Results
|
Specimen
2A/B:
embryonic day 16.5
(more )
Note: Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
Specimen
2A/B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lateral ganglionic eminence |
Present |
Regionally restricted |
2A
,
2B
|
Expressed in the dorsal region, mainly in the subventricular zone with increasing expression from anterior to posterior.
|
TS24: cerebral cortex subventricular zone |
Present |
|
2A
,
2B
|
|
TS24: neocortex |
Present |
Regionally restricted |
2A
,
2B
|
There was a migratory stream from the dorsal lateral ganglionic eminence to the neocortex through the subventricular zone. The majority of cells in the stream were Nr2f2+Sp8+.
|
Specimen
2E/F:
embryonic day 16.5
(more )
Note: Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
Specimen
2E/F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lateral ganglionic eminence |
Present |
Regionally restricted |
2F
,
2E
|
Expressed in the dorsal region, mainly in the subventricular zone with increasing expression from anterior to posterior.
|
TS24: medial ganglionic eminence |
Present |
Regionally restricted |
2F
,
2E
|
Expressed in the dorsal region, mainly in the subventricular zone with increasing expression from anterior to posterior.
|
TS24: neocortex |
Present |
Regionally restricted |
2F
,
2E
|
There was a migratory stream from the dorsal lateral ganglionic eminence to the neocortex through the subventricular zone. The majority of cells in the stream were Nr2f2+Sp8+.
|
TS24: cerebral cortex subventricular zone |
Present |
|
2F
,
2E
|
|
Specimen
2I/J:
embryonic day 16.5
(more )
Note: Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
Specimen
2I/J:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: magenta - Nr2f2; green - Sp8 (assay MGI:6490538); blue - Nkx2-1 (assay MGI:6490532).
|
Specimen
4A/B/C/D/E:
postnatal day 28
(more )
Note: Double labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541).
Specimen
4A/B/C/D/E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541).
|
Specimen
4F/G:
postnatal day 28
(more )
Note: Double labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541).
Specimen
4F/G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541).
|
Specimen
4H/I:
postnatal day 28
(more )
Note: Triple labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541); blue - Calb2 (assay MGI:6490544).
Specimen
4H/I:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: magenta - Nr2f2; green - Sst (assay MGI:6490541); blue - Calb2 (assay MGI:6490544).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
4I
,
4H
|
Nr2r2+Sst+ interneurons were mainly in layer V. No Nr2r2+Sst+Calb2+ neocortical interneurons.
|
Specimen
5A/B:
postnatal day 0
(more )
Note: Triple labeled: magenta - Nr2f2; blue - Sp8 (assay MGI:6490539); green - Sox6 (assay MGI:6490545).
Specimen
5A/B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: magenta - Nr2f2; blue - Sp8 (assay MGI:6490539); green - Sox6 (assay MGI:6490545).
|
Specimen
6A/C:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E12.5 and sacrificed at P28.
Specimen
6A/C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E12.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
6C
,
6A
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5.
|
Specimen
6B/D:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E14.5 and sacrificed at P28.
Specimen
6B/D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E14.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer I |
Present |
|
6D
,
6B
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5.
|
TS28: cortical layer II |
Present |
|
6D
,
6B
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5.
|
TS28: cortical layer III |
Present |
|
6D
,
6B
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5.
|
Specimen
7E:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * CD-1
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+ Shhtm2(cre/ERT2)Cjt/Shh+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: magenta - Nr2f2; green - green - Cre reporter (Shh).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
7E
|
Not expressed in the cell stream migrating from the preoptic area and ventral medial ganglionic eminence into the lateral ganglionic eminence.
|
Specimen
7J/L:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6J * CD-1
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+ Shhtm2(cre/ERT2)Cjt/Shh+
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: magenta - Nr2f2; green - green - Cre reporter (Shh).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: neocortex |
Present |
|
7L
,
7J
|
No expression in the pyramidal neurons migrated from the preoptic area and the medial ganglionic eminence (Shh-lineage).
|
Specimen
8A/B/E/F:
(close )
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6 * C57BL/6J * CD-1
|
Mutant Allele(s):
|
Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+ Tg(Nkx2-1-cre)2Sand/0
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Triple labeled: magenta - Nr2f2; blue - Sst (assay MGI:6490542); green - Cre reporter (Nkx2-1).
|
Specimen
not shown:
embryonic day 13.5
(more )
Note: Double labeled with histone H3.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled with histone H3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: medial ganglionic eminence |
Present |
Regionally restricted |
|
Nr2f2+pH3+ cells were seen in the ventricular zone of the dorsal region.
|
Specimen
not shown:
embryonic day 16.5
(more )
Note: Double labeled with histone H3.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled with histone H3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lateral ganglionic eminence |
Present |
Regionally restricted |
|
No Nr2f2+pH3+ cells were seen in the subventricular zone of the dorsal region.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E10.5 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E10.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E11.5 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E11.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E13.5 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E13.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E15.5 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E15.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E16.5 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E16.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E17.5 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E17.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at E18.5 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at E18.5 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
Specimen
not shown:
postnatal day 28
(more )
Note: Summarized in Fig 6E&6F. A single BrdU injection was given at P0 and sacrificed at P28.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 28
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Summarized in Fig 6E&6F. A single BrdU injection was given at P0 and sacrificed at P28.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cortical layer V |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E11.5 and E13.5 with a peak at E12.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer I |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer II |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
TS28: cortical layer III |
Present |
|
|
The majority of Nr2f2+ interneurons were born between E13.5 and E15.5 with a peak at E14.5 in both the motor and somatosensory cortex.
|
|