Antibody:	anti-Prcd
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present		1B , 1D	Expression is seen exclusively in the photoreceptor outer segments; double labeling demonstrates this includes expression in the rods.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present		1G , 1E	Expression is seen exclusively in the photoreceptor outer segments; the high background of the rod labeling made it difficult to determine if this included expression in the cones.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present		1H , 1J	Expression is seen in the photoreceptor outer segments; since these mutants lack rods, this demonstrates there is expression in the cones.

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Present		3E wt

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Absent		3E ko