Prcd^{tm1.1(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Prcd^{tm1.1(KOMP)Mbp}
• Name: photoreceptor disc component; targeted mutation 1.1, Mouse Biology Program, UC Davis
• MGI ID: MGI:6469333
• Gene: Prcd Location: Chr11:116544603-116559224 bp, + strand Genetic Position: Chr11, 81.46 cM
• Alliance: Prcd^{tm1.1(KOMP)Mbp} page
• IMPC: Prcd gene page

Mutation origin

• Mutant Cell Line: DEPD00545_2_A10
• Germline Transmission: Earliest citation of germline transmission: J:293626
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 2793 starting at position 116547922 and ending at position 116550715 of Chromosome 11 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon 1-4. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Cre-mediated recombination removed the selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:293626)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prcd Mutation: 8 strains or lines available

References

• Original: J:293626 Allon G, et al., PRCD is concentrated at the base of photoreceptor outer segments and is involved in outer segment disc formation. Hum Mol Genet. 2019 Dec 15;28(24):4078-4088
• All: 1 reference(s)