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Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467823
Gene symbol: Afdn
Gene name: afadin, adherens junction formation factor
Antibody: Anti-l/s-Afadin (A0224)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen S2A f/f left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Afdn; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: renal vesicle Present S2A upper left
Specimen S2A f/f right: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Afdn; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A upper right
Specimen S2A cKO left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: red - Afdn; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: renal vesicle Present S2A lower left , S2A lower left inset Similar as in the control.
Specimen S2A cKO right: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: red - Afdn; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A lower right , S2A lower right inset Variably decreased expression compared with the control.
Specimen S3Ba: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: red - Afdn; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: urothelium of ureter Present S3Ba right , S3Ba left
Specimen S3Bb: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Afdn; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: urothelium of ureter Weak S3Bb right , S3Bb left Reduced expression (in nearly half of all ureteric tubules) compared with the control.
Specimen S3Bc: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: red - Afdn; green - Ncam1 (assay MGI:6467814).
Structure Level Pattern Image Note
TS27: urothelium of ureter Absent S3Bc right , S3Bc left In about half of all ureteric tubules.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory