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Gene Expression Data
Assay Details
Assay
Reference: J:245763 Gao L, et al., Afadin orients cell division to position the tubule lumen in developing renal tubules. Development. 2017 Oct 01;144(19):3511-3520
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6467814
Gene symbol: Ncam1
Gene name: neural cell adhesion molecule 1
Antibody: 5B8 antibody
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 5A/A': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: green - Ncam1; red - alpha-tubulin; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 5A , 5A' Expressed in the basolateral junction. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5B/B': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: green - Ncam1; red - alpha-tubulin; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 5B , 5B' Expressed in the basolateral junction. The orientation of division appeared to be parallel to the luminal/apical surface.
Specimen 5C/C': embryonic day 17.5; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Triple labeled: green - Ncam1; red - alpha-tubulin; blue - Pard6b (assay MGI:6467789).
Structure Level Pattern Image Note
TS25: s-shaped body Present 5C , 5C' Expressed in the basolateral junction. The midbody was formed apically.
Specimen S2A f/f left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A upper left Expressed in the basolateral junction.
Specimen S2A f/f right: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A upper right Expressed in the basolateral junction.
Specimen S2A cKO left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A lower left Expressed in the basolateral junction.
Specimen S2A cKO right: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2A lower right Expressed in the basolateral junction.
Specimen S2B f/f left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2B upper left Expressed in the basolateral junction.
Specimen S2B cKO left: postnatal day 0; Afdntm1.1Lfr/Afdntm1.1Lfr, Wnt4tm3(EGFP/cre)Amc/Wnt4+ (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S2B lower left Expressed in the basolateral junction.
Specimen S3Ba: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr (more )
Note: Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S3Ba right Expressed in the basolateral junction.
Specimen S3Bb: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S3Bb right Expressed in the basolateral junction.
Specimen S3Bc: postnatal newborn; Afdntm1.1Lfr/Afdntm1.1Lfr, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: green - Ncam1; red - Afdn (assay MGI:6467823).
Structure Level Pattern Image Note
TS27: s-shaped body Present S3Bc right Expressed in the basolateral junction.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory