Afdn^tm1.1Lfr
Targeted Allele Detail

Summary

• Symbol: Afdn^tm1.1Lfr
• Name: afadin, adherens junction formation factor; targeted mutation 1.1, Louis F Reichardt
• MGI ID: MGI:5308214
• Synonyms: afadin flox
• Gene: Afdn Location: Chr17:13980735-14126059 bp, + strand Genetic Position: Chr17, 8.95 cM, cytoband A2
• Alliance: Afdn^tm1.1Lfr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:179366
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 2 and an FRT flanked neomycin selection cassette and second loxP site were inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neomycin selection cassette leaving exon 2 floxed. (J:179366)

Expression

In Mice Carrying this Mutation:	53 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Afdn Mutation: 84 strains or lines available

References

• Original: J:179366 Beaudoin GM 3rd, et al., Afadin, a ras/rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus. J Neurosci. 2012 Jan 4;32(1):99-110
• All: 10 reference(s)