Tbx3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:272619 Aydogdu N, et al., TBX2 and TBX3 act downstream of canonical WNT signaling in patterning and differentiation of the mouse ureteric mesenchyme. Development. 2018 Nov 26;145(23):dev171827
Assay type:	RNA in situ
MGI Accession ID:	MGI:6385390
Gene symbol:	Tbx3
Gene name:	T-box 3

Probe:	Tbx3 probe12
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen 1A E12.5: embryonic day 12.5 (more

)
Note: proximal (kidney) level

Structure	Level	Pattern	Image	Note
TS20: epithelial layer of ureter	Strong		1A E12.5 Tbx3
TS20: ureteral mesenchyme subepithelial layer	Present		1A E12.5 Tbx3

Specimen 1A E14.5: embryonic day 14.5 (more

)
Note: proximal (kidney) level

Structure	Level	Pattern	Image	Note
TS22: urothelium of ureter	Strong		1A E14.5 Tbx3
TS22: ureteral mesenchyme subepithelial layer	Present		1A E14.5 Tbx3

Specimen 1A E16.5: embryonic day 16.5 (more

)
Note: proximal (kidney) level

Structure	Level	Pattern	Image	Note
TS24: urothelium of ureter	Strong		1A E16.5 Tbx3
TS24: ureteral mesenchyme subepithelial layer	Present		1A E16.5 Tbx3

Specimen 1A E18.5: embryonic day 18.5 (more

)
Note: proximal (kidney) level

Structure	Level	Pattern	Image	Note
TS26: urothelium of ureter	Strong		1A E18.5 Tbx3

Specimen 1C control: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: epithelial layer of ureter	Strong		1C control Tbx3
TS20: ureteral mesenchyme subepithelial layer	Present		1C control Tbx3

Specimen 1C Tbx18^cre/+;Ctnnb1^fl/fl: embryonic day 12.5; Ctnnb1^tm2Kem/Ctnnb1^tm2Kem, Tbx18^tm4(cre)Akis/Tbx18⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: epithelial layer of ureter	Strong		1C Tbx18^cre/+;Ctnnb1^fl/fl Tbx3
TS20: ureteral mesenchyme subepithelial layer	Absent		1C Tbx18^cre/+;Ctnnb1^fl/fl Tbx3

Specimen 1C Tbx18^cre/+;Ctnnb1^(ex3)fl/+: embryonic day 12.5; Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt, Tbx18^tm4(cre)Akis/Tbx18⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: epithelial layer of ureter	Strong		1C Tbx18^cre/+;Ctnnb1^(ex3)fl/+ Tbx3
TS20: ureteral mesenchyme	Present		1C Tbx18^cre/+;Ctnnb1^(ex3)fl/+ Tbx3	Misexpression of a stabilized version of Ctnnb1 in the entire ureteric mesenchyme resulted in increased and expanded expression of Tbx3.

Specimen 1C Tbx18^cre/+;Smo^fl/fl: embryonic day 12.5; Smo^tm2Amc/Smo^tm2Amc, Tbx18^tm4(cre)Akis/Tbx18⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: epithelial layer of ureter	Strong		1C Tbx18^cre/+;Smo^fl/fl Tbx3
TS20: ureteral mesenchyme subepithelial layer	Present		1C Tbx18^cre/+;Smo^fl/fl Tbx3	Loss of Smo had no effect on Tbx3 expression in the ureteric mesenchyme at E12.5.