Probe:	C530008M17Rik cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present		2A
TS12: thoracic segment of trunk	Present	Regionally restricted	2A	Expression was detected in the second heart field/first branchial arch/outflow tract region where the primitive tubular heart attaches to the pharyngeal foregut.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present		2B
TS14: neural tube	Present		2B
TS14: prosencephalon	Present		2B
TS14: thoracic segment of trunk	Present	Regionally restricted	2B	Expression was detected in the second heart field/first branchial arch/outflow tract region.
TS14: pericardium	Present	Regionally restricted	2B	Expression was detected in the pericardial cavity adjacent to the outflow tract.

Structure	Level	Pattern	Image	Note
TS22: brain	Present		2C
TS22: dorsal root ganglion	Present		2C
TS22: hindbrain	Present		2C
TS22: spinal cord	Present		2C

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Widespread	2D	Expression was detected throughout the neural tube, except in the floor plate.
TS16: neural tube floor plate	Absent		2D

Structure	Level	Pattern	Image	Note
TS18: neural tube	Present	Widespread	2E	Expression was detected throughout the neural tube, except in the floor plate.
TS18: neural tube floor plate	Absent		2E
TS18: dorsal root ganglion	Present		2E

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Widespread	2F	Expression was detected throughout the neural tube, except in the floor plate.
TS20: spinal cord floor plate	Absent		2F
TS20: dorsal root ganglion	Present		2F
TS20: sinus venosus	Present		2F
TS20: sympathetic ganglion	Present		2F

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present		2H	Expression was at a reduced level compared to earlier stages.
TS22: spinal cord marginal layer	Absent		2H
TS22: spinal cord roof plate	Absent		2H
TS22: dorsal root ganglion	Present		2H
TS22: sympathetic ganglion	Present		2H
TS22: vagal nerve trunk	Present		2H
TS22: lung	Present	Punctate	2H

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Weak	Regionally restricted	2I	Expression in the spinal cord was only in the mantle layer and was at a reduced level compared to earlier stages.
TS27: spinal cord marginal layer	Absent		2I
TS27: dorsal root ganglion	Present		2I
TS27: sympathetic ganglion	Present		2I

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		2K
TS27: hippocampus	Present		2K
TS27: main olfactory bulb	Present		2K

Structure	Level	Pattern	Image	Note
TS15: glossopharyngeal preganglion	Present		3A
TS15: neural tube	Present		3A
TS15: secondary heart field	Present		3A
TS15: sinus venosus	Present		3A
TS15: pericardium	Present	Regionally restricted	3A	Expression was detected in the dorsal pericardial wall that is contiguous with the outflow myocardium.

Structure	Level	Pattern	Image	Note
TS17: outflow tract cardiac muscle	Present	Regionally restricted	3B	Expression was detected in the outflow tract/secondary heart field region in the cardiomyocyte outer layer.
TS17: primitive ventricle	Present	Regionally restricted	3B	Expression was detected in the right ventricle in the cardiomyocyte outer layer.
TS17: 2nd branchial arch mesenchyme	Present		3B
TS17: trunk mesenchyme	Present	Regionally restricted	3B	Expression was detected in mesenchyme between cardinal veins and dorsal aorta.

Structure	Level	Pattern	Image	Note
TS17: heart atrium	Present	Regionally restricted	3D	Expression was detected in the inflow region, in the walls of the atria.
TS17: foregut	Present	Regionally restricted	3D	Expression was detected in the foregut endoderm.
TS17: common atrial chamber	Present		3D
TS17: sinus venosus	Present		3D

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		3E
TS20: sinus venosus	Present		3E
TS20: spinal cord	Present		3E
TS20: sympathetic ganglion	Present		3E

Structure	Level	Pattern	Image	Note
TS22: vena cava	Present	Regionally restricted	3F	Expression was detected in the wall of the vena cava just before it enters the right atrium.

Structure	Level	Pattern	Image	Note
TS28: atrium cardiac muscle	Present	Punctate	3G

Structure	Level	Pattern	Image	Note
TS28: atrium cardiac muscle	Present	Punctate	3H
TS28: heart endothelium	Absent		3H

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Strong		4A	Very robust expression was detected in mitotic spermatogonia and meiotic primary spermatocytes Type A and B.
TS28: spermatogonium	Strong		4A	Very robust expression was detected in mitotic spermatogonia and meiotic primary spermatocytes Type A and B.
TS28: spermatid	Absent		4A
TS28: spermatozoon	Absent		4A

Structure	Level	Pattern	Image	Note
TS28: ovary	Absent

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Strong		4B	Very robust expression was detected in mitotic spermatogonia and meiotic primary spermatocytes Type A and B.
TS28: spermatogonium	Strong		4B	Very robust expression was detected in mitotic spermatogonia and meiotic primary spermatocytes Type A and B.
TS28: spermatid	Absent		4B
TS28: spermatozoon	Absent		4B

Structure	Level	Pattern	Image	Note
TS22: intestine mesenchyme	Present	Punctate	4D	Expression was detected in close apposition to the endoderm.

Structure	Level	Pattern	Image	Note
TS27: crypt of lieberkuhn	Present		4E

Structure	Level	Pattern	Image	Note
TS27: crypt of lieberkuhn	Present		4F

Structure	Level	Pattern	Image	Note
TS28: crypt of lieberkuhn	Present		4H

Structure	Level	Pattern	Image	Note
TS27: epidermis	Weak		4I	Limited expression was present in abdominal newborn skin epidermis.
TS27: hair root sheath	Strong		4I
TS27: connective tissue	Strong	Regionally restricted	4I	Robust expression was detected in connective tissue/bulge surrounding the hair follicles.
TS27: dermis	Absent		4I
TS27: hair follicle dermal papilla	Absent		4I

Structure	Level	Pattern	Image	Note
TS27: brown fat	Present	Punctate	4J	Expression was punctate in brown adipose and fibrous septae.

Structure	Level	Pattern	Image	Note
TS28: white fat	Present		4K

Structure	Level	Pattern	Image	Note
TS28: testis	Present		5C

Structure	Level	Pattern	Image	Note
TS28: testis	Weak		5D	Homozygous mutant testis exhibits significantly reduced mRNA levels compared to controls, despite similar patterns of expression.