Gene Expression Image Detail

Reference

Figure

ID

J:278327 Snider PL, et al., J Cardiovasc Dev Dis. 2019 Jun 22;6(2)

3

MGI:6378728

Image

Caption

(A) Whole mount RNA in situ hybridization at E9.5. Staining in the second heart field (arrow) and in glossopharyngeal ganglia (arrowhead). Note staining in otic vesicle (*) is artefactual trapping. (B,C) In situ hybridization (B) and immunostaining (C) in adjacent transversely sectioned E10.5 embryo. Overlapping staining was noted in the outflow tract/second heart field region and right ventricle (rv), specifically in the cardiomyocyte outer layer (arrowheads (B,C)). Also, staining in the second branchial arch (2nd) and in mesenchyme (arrows B) between cardinal veins and immunopositive dorsal aorta (arrows (C)). (D) Staining in E10.5 inflow region, in the walls of the atria (arrowheads) and foregut (fore) endoderm. (E,F) Staining is in both left and right horns of the E12.5 sinus venosus (* in (E)), neural tube, sympathetic ganglia (arrows), and dorsal root ganglia (drg) in transverse sections. Sagittal E14 wall of the vena cava (vc) just before it enters the right atrium (arrow (F)). (G,H) Staining in adult is in a punctate pattern amongst myocytes (arrowheads,(H)) but is absent from overlying endothelial lineage (arrow). (I) In situ hybridization negative sense control. Scale bars: (B,C) = 50 um; (D) = 20 um; (E-H) = 100 um. Abbreviations: 1st, first branchial arch; a, atria; bw, thoracic body wall; lb, limb bud; lj, lower jaw; nt, neural tube; ra, right atria; rv, right ventricle; vent, ventricle.

Copyright

This image is Snider PL, J Cardiovasc Dev Dis 2019 Jun 22;6(2):, and is displayed under the terms of the Creative Commons Attribution 4.0 International License.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A	MGI:6378757 (Cracd)
B	MGI:6378757 (Cracd)
C	MGI:6378759 (Acta2)
D	MGI:6378757 (Cracd)
E	MGI:6378757 (Cracd)
F	MGI:6378757 (Cracd)
G	MGI:6378757 (Cracd)
H	MGI:6378757 (Cracd)