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Gene Expression Data
Assay Details
Assay
Reference: J:273053 Shih HP, et al., A Gene Regulatory Network Cooperatively Controlled by Pdx1 and Sox9 Governs Lineage Allocation of Foregut Progenitor Cells. Cell Rep. 2015 Oct 13;13(2):326-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361315
Gene symbol: Pdx1
Gene name: pancreatic and duodenal homeobox 1
Antibody: Anti-Pdx1
Detection system: Secondary antibody coupled to Cy5
Assay notes: Two antibodies were described, but the one used in this assay was not specified; either MGI:6361200 or MGI:6361201 could have been used.
Results
Specimen 3B: embryonic day 10.5 (more )
Note: Double labeled: red - Pdx1; green - Foxa2 (assay MGI:6361336).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 3B' , 3B
TS17: pancreas primordium ventral bud Present 3B' , 3B
Specimen 3D: embryonic day 10.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Zp3-cre)93Knw/0 (more )
Note: Conditional mutant. It was unclear what Cre allele was used; it is possible Prm1-Cre MGI:2176182 was used. Double labeled: red - Pdx1; green - Foxa2 (assay MGI:6361336).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present 3D' , 3D Staining intensity was reduced in the mutant.
TS17: pancreas primordium ventral bud Present 3D' , 3D Staining intensity was reduced in the mutant.
Specimen 3E: embryonic day 9.5; Sox9tm1Gsr/Sox9tm1Gsr (more )
Note: Double labeled: red - Pdx1; green - Sox9 (assay MGI:6361337).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Present 3E , 3E''
Specimen 3F: embryonic day 9.5; Sox9tm1Gsr/Sox9tm1Gsr (more )
Note: Double labeled: red - Pdx1; green - Sox9 (assay MGI:6361337).
Structure Level Pattern Image Note
TS15: pancreas primordium ventral bud Present 3F , 3F''
Specimen 3G: embryonic day 9.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: red - Pdx1; green - Sox9 (assay MGI:6361337).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Present 3G'' , 3G
Specimen 3H: embryonic day 9.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: red - Pdx1; green - Sox9 (assay MGI:6361337).
Structure Level Pattern Image Note
TS15: pancreas primordium ventral bud Present 3H'' , 3H
Specimen 6A: embryonic day 10.5 (more )
Note: Double labeled: red - Pdx1; green - Cdx2 (assay MGI:6361261).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Strong 6A'' , 6A
TS17: foregut-midgut junction Strong Regionally restricted 6A'' , 6A At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6B: embryonic day 10.5; Sox9tm1Gsr/Sox9+, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: red - Pdx1; green - Cdx2 (assay MGI:6361261).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Strong 6B'' , 6B
TS17: foregut-midgut junction Strong Regionally restricted 6B , 6B'' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6C: embryonic day 10.5; Pdx1tm2Cvw/Pdx1+ (more )
Note: Double labeled: red - Pdx1; green - Cdx2 (assay MGI:6361261).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Strong 6C'' , 6C
TS17: foregut-midgut junction Strong Regionally restricted 6C'' , 6C At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6D: embryonic day 10.5; Pdx1tm2Cvw/Pdx1+, Sox9tm1Gsr/Sox9+, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: red - Pdx1; green - Cdx2 (assay MGI:6361261).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Strong 6D'' , 6D
TS17: foregut-midgut junction Strong Regionally restricted 6D , 6D'' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6E: embryonic day 10.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: red - Pdx1; green - Cdx2 (assay MGI:6361261).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Strong 6E , 6E''
TS17: foregut-midgut junction Strong Regionally restricted 6E'' , 6E At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6F: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw (more )
Note: Double labeled: red - Pdx1; green - Cdx2 (assay MGI:6361261).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Strong 6F'' , 6F The antibody used recognized the truncated Pdx1 protein expressed in the Pdx1 null allele.
TS17: foregut-midgut junction Strong Regionally restricted 6F , 6F'' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6G: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: red - Pdx1; green - Cdx2 (assay MGI:6361261).
Structure Level Pattern Image Note
TS17: foregut Strong Regionally restricted 6G'' , 6G The antibody used recognized the truncated Pdx1 protein expressed in the Pdx1;Sox9 mutant allele.
TS17: foregut-midgut junction Strong Regionally restricted 6G'' , 6G Loss of both Pdx1 and Sox9 results in ectopic Cdx2 expression. There was extensive overlap between the Pdx1 and Cdx2 domains.
Specimen S1A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1A
Specimen S1B: embryonic day 10.5; Sox9tm1Gsr/Sox9+, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1B
Specimen S1C: embryonic day 10.5; Pdx1tm2Cvw/Pdx1+ (more )
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1C
Specimen S1D: embryonic day 10.5; Pdx1tm2Cvw/Pdx1+, Sox9tm1Gsr/Sox9+, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1D
Specimen S1E: embryonic day 10.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1E
Specimen S1F: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw (more )
Structure Level Pattern Image Note
TS17: pancreas primordium Present S1F The antibody used recognized the truncated Pdx1 protein expressed in the Pdx1 null allele.
Specimen S1G: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: foregut Present S1G The antibody used recognized the truncated Pdx1 protein expressed in the Pdx1;Sox9 mutant allele.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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