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Gene Expression Data
Assay Details
Assay
Reference: J:203015 Panayiotou E, et al., Pax6 is expressed in subsets of V0 and V2 interneurons in the ventral spinal cord in mice. Gene Expr Patterns. 2013 Dec;13(8):328-34
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6360666
Gene symbol: Pax6
Gene name: paired box 6
Antibody: Anti-Pax6
Assay notes: Two Pax6 antibodies were described MGI:6356544 and MGI:6356545; either could have been used in this assay.
Results
Specimen 2B: embryonic day 14.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Dbx1-icre)1Wdr/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 2B , 2C Expression was detected in the Foxn4 lineage.
Specimen 2E: embryonic day 14.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Foxn4-icre)1Smal/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 2F , 2E About 25% of Pax6-positive interneurons originated from Foxn4 progenitors suggesting origin from the p2 domain.
Specimen 2H: embryonic day 14.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Olig2tm1.1(cre)Wdr/Olig2+ (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 2H , 2I No Pax6 positive interneurons originated from the Olig2 lineage (p3/pMN/ventral-p2 domain).
Specimen 3B: embryonic day 14.5 (more )
Note: Double labeled: red - Pax6; green - Vsx2 (assay MGI:6360726). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 3B , 3C Expression was detected in interneurons. There was no co-expression with Vsx2 positive V2a interneurons.
Specimen 3E: embryonic day 14.5 (more )
Note: Double labeled: red - Pax6; green - Gata3 (assay MGI:6360727). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 3E , 3F Expression was detected in interneurons. There was some co-expression with Gata3 positive V2b interneurons. Only the Pax6 interneurons settled in the ventral spinal cord coexpressed Gata3, and not the Pax6 interneurons when they migrate out of the ventricular zone.
Specimen 3H: embryonic day 14.5 (more )
Note: Double labeled: red - Pax6; green - Foxp2 (assay MGI:6360728). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 3I , 3H Expression was detected in interneurons.
Specimen 3K: embryonic day 14.5 (more )
Note: Double labeled: red - Pax6; green - Evx1 (assay MGI:6360729). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 3K , 3L Expression was detected in interneurons. There was some co-expression with Evx1 positive V0 interneurons.
Specimen 3N: embryonic day 14.5 (more )
Note: Double labeled: red - Pax6; green - Pax2 (assay MGI:6360730). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 3O , 3N Expression was detected in interneurons. There was some co-expression with Pax2 positive ventral V0 interneurons.
Specimen 4C: embryonic day 14.5 (more )
Note: Triple labeled: blue - Pax6; green - Pax2 (assay MGI:6360730); red - Gata3 (assay MGI:6360727). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 4D , 4C Expression was detected in interneurons. There was some co-expression with Pax2 positive ventral V0 interneurons and with Gata3 positive V2b interneurons. There were also Pax6 positive interneurons that do not express either Pax2 or Gata3.
Specimen S1B: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Foxn4-icre)1Smal/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted S1C , S1B The lineage relationship between Foxn4-expressing progenitors and Pax6 interneurons continued to hold true at a range of developmental stages.
Specimen S1E: embryonic day 15.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Foxn4-icre)1Smal/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS23: spinal cord Present Regionally restricted S1F , S1E The lineage relationship between Foxn4-expressing progenitors and Pax6 interneurons continued to hold true at a range of developmental stages.
Specimen S1H: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Foxn4-icre)1Smal/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS25: spinal cord Present Regionally restricted S1I , S1H The lineage relationship between Foxn4-expressing progenitors and Pax6 interneurons continued to hold true at a range of developmental stages.
Specimen S1K: postnatal day 4; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Foxn4-icre)1Smal/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: spinal cord Present Regionally restricted S1K , S1L The lineage relationship between Foxn4-expressing progenitors and Pax6 interneurons continued to hold true at a range of developmental stages.
Specimen S1N: postnatal day 25; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Foxn4-icre)1Smal/0 (more )
Note: Conditional mutant. Double labeled: red - Pax6; green - Gt(ROSA)26Sor reporter (assay MGI:6360698). Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS28: spinal cord Present Regionally restricted S1N , S1O The lineage relationship between Foxn4-expressing progenitors and Pax6 interneurons continued to hold true into adulthood.
Specimen S2B: embryonic day 14.5 (more )
Note: Double labeled: red - Pax6; green - Glutamate. Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted S2D , S2B , S2C Pax6-positive interneurons were heterogeneous; some were glutamatergic (excitatory).
Specimen S2F: embryonic day 14.5 (more )
Note: Double labeled: red - Pax6; green - Glycine. Specimen was fixed in MEMFA or 4% paraformaldehyde.
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted S2H , S2G , S2F Pax6-positive interneurons were heterogeneous; some were glycinergic (inhibatory).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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