About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:271100 Shen B, et al., Genetic dissection of tie pathway in mouse lymphatic maturation and valve development. Arterioscler Thromb Vasc Biol. 2014 Jun;34(6):1221-30
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6314707
Gene symbol: Prox1
Gene name: prospero homeobox 1
Results
Specimen 2A ICD/ICD Prox-1: embryonic day 12.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Brdu.
Structure Level Pattern Image Note
TS20: lymph sac Present Regionally restricted 2A ICD/ICD Overlay , 2A ICD/ICD Prox-1
Specimen 2A +/ICD Prox-1: embryonic day 12.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Brdu.
Structure Level Pattern Image Note
TS20: lymph sac Present Regionally restricted 2A +/ICD Overlay , 2A +/ICD Prox-1 Expression in lymphatic endothelial cells of jugular lymph sacs.
Specimen 2A +/+ Prox-1: embryonic day 12.5 (more )
Note: Double labeled: red - Prox1; green - Brdu.
Structure Level Pattern Image Note
TS20: lymph sac Present Regionally restricted 2A +/+ Overlay , 2A +/+ Prox-1 Expression in lymphatic endothelial cells of jugular lymph sacs.
Specimen 2D ICD/ICD Prox-1: embryonic day 15.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Lyve1 (assay MGI:6314706).
Structure Level Pattern Image Note
TS23: lymphatic system Present Regionally restricted 2D ICD/ICD Prox-1 (right) , 2D ICD/ICD Overlay , 2D ICD/ICD Prox-1 (left) Authors report disruption of proper formation of lymphatic network compared to that of control mice.
Specimen 2D +/ICD Prox-1: embryonic day 15.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Lyve1 (assay MGI:6314706).
Structure Level Pattern Image Note
TS23: back skin Present Regionally restricted 2D +/ICD Prox-1 (right) , 2D +/ICD Prox-1 (left) , 2D +/ICD Overlay Expression in lymphatic endothelial cells.
Specimen 2D +/+ Prox-1: embryonic day 15.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6314706).
Structure Level Pattern Image Note
TS23: back skin Present Regionally restricted 2D +/+ Prox-1 (right) , 2D +/+ Overlay , 2D +/+ Prox-1 (left) Expression in lymphatic endothelial cells.
Specimen 3A Prox-1: embryonic day 18.5 (more )
Note: Double labeled: red - Prox1; green - Tie1 (assay MGI:6314704).
Structure Level Pattern Image Note
TS26: back skin Present Regionally restricted 3A Prox-1 , 3A Overlay Expression in lymphatic endothelial cells.
Specimen 3C ICD/ICD Prox-1: embryonic day 15.75; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Triple labeled: green - Prox1; red - Lyve1 (assay MGI:6314706); blue - Tie1 (assay MGI:6314704).
Structure Level Pattern Image Note
TS23: lymphatic system Present Regionally restricted 3C ICD/ICD Prox-1 , 3C ICD/ICD Overlay Authors report disruption of proper formation of lymphatic network compared to that of control mice.
Specimen 3C +/ICD Prox-1: embryonic day 15.75; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Triple labeled: green - Prox1; red - Lyve1 (assay MGI:6314706); blue - Tie1 (assay MGI:6314704).
Structure Level Pattern Image Note
TS23: lymphatic vessel Strong Regionally restricted 3C +/ICD Overlay , 3C +/ICD Prox-1 Authors report strong expression in a lymphatic vessel with weak Lyve1 expression.
Specimen 3D ICD/ICD Prox-1: embryonic day 18.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: lymphatic system Present Regionally restricted 3D ICD/ICD Prox1 , 3D ICD/ICD Overlay Authors report no formation of collecting lymphatic vessels and valves in homozygous mutant mice.
Specimen 3D +/ICD Prox-1: embryonic day 18.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: back skin Present Regionally restricted 3D +/ICD Overlay , 3D +/ICD Prox-1 Expression in lymphatic endothelial cells.
Specimen 3D +/+ Prox-1: embryonic day 18.5 (more )
Note: Double labeled: red - Prox1; green - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: back skin Present Regionally restricted 3D +/+ Overlay , 3D +/+ Prox-1 Expression in lymphatic endothelial cells.
Specimen 4B ICD/ICD Prox-1: embryonic day 17.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: green - Prox1; red - Tie1 (assay MGI:6314704).
Structure Level Pattern Image Note
TS25: lymphatic system Present Regionally restricted 4B ICD/ICD Overlay , 4B ICD/ICD Prox-1 Authors report disruption of remodeling of primary lymphatic network in homozygous mutant mice.
Specimen 4B +/ICD Prox-1: embryonic day 17.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: green - Prox1; red - Tie1 (assay MGI:6314704).
Structure Level Pattern Image Note
TS25: gut mesentery Present Regionally restricted 4B +/ICD Prox-1 , 4B +/ICD Overlay Expression in lymphatic endothelial cells.
Specimen 4B +/+ Prox-1: embryonic day 17.5 (more )
Note: Double labeled: green - Prox1; red - Tie1 (assay MGI:6314704).
Structure Level Pattern Image Note
TS25: gut mesentery Present Regionally restricted 4B +/+ Overlay , 4B +/+ Prox-1 Expression in lymphatic endothelial cells.
Specimen 4C ICD/ICD Prox-1: embryonic day 18.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Triple labeled: green - Prox1; red - Acta2 (assay MGI:6314709); blue - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: lymphatic system Present Regionally restricted 4C ICD/ICD Overlay , 4C ICD/ICD Prox-1 Authors report no formation of lymphatic valves in mesentery of homozygous mutants.
Specimen 4C +/ICD Prox-1: embryonic day 18.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Triple labeled: green - Prox1; red - Acta2 (assay MGI:6314709); blue - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: gut mesentery Present Regionally restricted 4C +/ICD Prox-1 , 4C +/ICD Overlay
Specimen 4C +/+ Prox-1: embryonic day 18.5 (more )
Note: Triple labeled: green - Prox1; red - Acta2 (assay MGI:6314709); blue - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: gut mesentery Present Regionally restricted 4C +/+ Prox-1 , 4C +/+ Overlay
Specimen 7D Ang2-/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Lyve1 (assay MGI:6314706).
Structure Level Pattern Image Note
TS26: lymphatic vessel Present 7D Ang2-/- Prox-1 , 7D Ang2-/- Overlay
Specimen 7D Ang2+/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Lyve1 (assay MGI:6314706).
Structure Level Pattern Image Note
TS26: lymphatic vessel Present 7D Ang2+/- Overlay , 7D Ang2+/- Prox-1
Specimen 7G Ang2-/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: lymphatic system Present Regionally restricted 7G Ang2-/- Overlay , 7G Ang2-/- Prox-1 Authors report no formation of collecting lymphatic vessels and valves in homozygous mutant mice.
Specimen 7G Ang2+/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: red - Prox1; green - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: lymphatic vessel Present 7G Ang2+/- Overlay , 7G Ang2+/- Prox-1
Specimen 8A Prox-1: postnatal; Angpt2tm1.1Yuhe/Angpt2+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Triple labeled: green - Prox1; red - Tek (assay MGI:6314710); blue - Hoechst.
Structure Level Pattern Image Note
TS28: lymphatic system Present 8A Overlay , 8A Prox-1
Specimen S2 E14.5 ICD/ICD: embryonic day 14.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: lymphatic system Present S2_ICD/ICD E14.5
Specimen S2 E14.5 +/ICD: embryonic day 14.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: lymphatic system Present S2_WT/ICD E14.5
Specimen S2 E14.5 +/+: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: lymphatic system Present S2_WT/WT E14.5
Specimen S2 E15.5 ICD/ICD: embryonic day 15.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: lymphatic system Present S2_ICD/ICD E15.5
Specimen S2 E15.5 +/ICD: embryonic day 15.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: lymphatic system Strong Regionally restricted S2_WT/ICD E15.5 Strong expression as valve morphogenesis is initiated with formation of lymphatic endothelial cell (LEC) clusters.
Specimen S2 E15.5 +/+: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: lymphatic system Strong Regionally restricted S2_WT/WT E15.5 Strong expression as valve morphogenesis is initiated with formation of lymphatic endothelial cell (LEC) clusters.
Specimen S2 E16.5 ICD/ICD: embryonic day 16.5; Tie1tm1.1Yuhe/Tie1tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: lymphatic system Present S2_ICD/ICD E16.5
Specimen S2 E16.5 +/ICD: embryonic day 16.5; Tie1tm1.1Yuhe/Tie1+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: lymphatic system Strong Regionally restricted S2_WT/ICD E16.5 Expression becomes more obvious in valve forming regions with condensed lymphatic endothelial cells.
Specimen S2 E16.5 +/+: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: lymphatic system Strong Regionally restricted S2_WT/WT E16.5 Expression becomes more obvious in valve forming regions with condensed lymphatic endothelial cells.
Specimen S3A Ang2-/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: green - Prox1; red - Acta2 (assay MGI:6314709).
Structure Level Pattern Image Note
TS26: lymphatic system Present Regionally restricted S3A Ang2-/- Prox1 , S3A Ang2-/- Overlay Authors report remodeling of primary lymphatic network to form collecting vessels does not occur in homozygous mutant mice.
Specimen S3A Ang2+/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: green - Prox1; red - Acta2 (assay MGI:6314709).
Structure Level Pattern Image Note
TS26: gut mesentery Present S3A Ang2+/- Overlay , S3A Ang2+/- Prox1
Specimen S3A Ang2+/+ Prox-1: embryonic day 18.5 (more )
Note: Double labeled: green - Prox1; red - Acta2 (assay MGI:6314709).
Structure Level Pattern Image Note
TS26: gut mesentery Present S3A Ang2+/+ Prox1 , S3A Ang2+/+ Overlay
Specimen S3B Ang2-/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2tm1.1Yuhe, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: green - Prox1; red - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: lymphatic system Present Regionally restricted S3B Ang2-/- Prox1 , S3B Ang2-/- Overlay Authors report no formation of lymphatic valves in mesentery of homozygous mutant mice.
Specimen S3B Ang2+/- Prox-1: embryonic day 18.5; Angpt2tm1.1Yuhe/Angpt2+, Tg(EIIa-cre)C5379Lmgd/0 (more )
Note: Conditional mutant. Double labeled: green - Prox1; red - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: gut mesentery Present S3B Ang2+/- Prox1 , S3B Ang2+/- Overlay
Specimen S3B Ang2+/+ Prox-1: embryonic day 18.5 (more )
Note: Double labeled: green - Prox1; red - Itga9 (assay MGI:6314708).
Structure Level Pattern Image Note
TS26: gut mesentery Present S3B Ang2+/+ Overlay , S3B Ang2+/+ Prox1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory