Results
|
Specimen
3B 15c:
embryonic day 3.0; Spry4tm1Hadj/Spry4 +
(more )
Note: 15 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
3B 15c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
15 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
3B 30c:
embryonic day 3.5; Spry4tm1Hadj/Spry4 +
(more )
Note: 30 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
3B 30c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
30 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
3B 57c:
embryonic day 3.5; Spry4tm1Hadj/Spry4 +
(more )
Note: 57 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
3B 57c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
57 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: inner cell mass |
Present |
Non-Uniform |
3B 57c alpha-GFP
,
3B 57c Merge + Nuclei
|
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
|
Specimen
3B 86c:
embryonic day 3.75; Spry4tm1Hadj/Spry4 +
(more )
Note: 86 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
3B 86c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.75
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
86 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: inner cell mass |
Present |
Non-Uniform |
3B 86c Merge + Nuclei
,
3B 86c alpha-GFP
|
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
|
Specimen
3B 91c:
embryonic day 4.0; Spry4tm1Hadj/Spry4 +
(more )
Note: 91 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
3B 91c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 4.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
91 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Structure |
Level |
Pattern |
Image |
Note |
TS5: inner cell mass |
Present |
Non-Uniform |
3B 91c alpha-GFP
,
3B 91c Merge + Nuclei
|
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
|
Specimen
3B 141c:
embryonic day 4.5; Spry4tm1Hadj/Spry4 +
(more )
Note: 141 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
3B 141c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 4.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
141 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Structure |
Level |
Pattern |
Image |
Note |
TS5: inner cell mass |
Present |
Non-Uniform |
3B 141c Merge + Nuclei
,
3B 141c alpha-GFP
|
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
|
Specimen
3B 159c:
embryonic day 5.0; Spry4tm1Hadj/Spry4 +
(more )
Note: 159 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
3B 159c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 5.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
159 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Structure |
Level |
Pattern |
Image |
Note |
TS5: inner cell mass |
Present |
Non-Uniform |
3B 159c alpha-GFP
,
3B 159c Merge + Nuclei
|
Expression increased as blastocysts developed. Expression in the inner cell mass (ICM) was heterogenous. Embryos were stained for Nanog and Gata6 to identify the epiblast (Nanog+), extraembryonic primitive endoderm (Gata6+), double positive, and double negative cell populations.
|
Specimen
not shown 16-32c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4tm1Hadj
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
16-32 cells
|
Specimen
not shown 32-64c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4tm1Hadj
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
32-64 cells
|
Specimen
not shown 64-90c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4tm1Hadj
|
Age:
|
embryonic day 3.75
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
64-90 cells
|
Specimen
not shown 90-120c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4tm1Hadj
|
Age:
|
embryonic day 4.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
90-120 cells
|
Specimen
not shown 120-150c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4tm1Hadj
|
Age:
|
embryonic day 4.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
120-150 cells
|
Specimen
not shown >150c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4tm1Hadj
|
Age:
|
embryonic day 5.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
>150 cells
|
Specimen
4A 58c:
embryonic day 3.5; Spry4tm1Hadj/Spry4 +
(more )
Note: 58 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
4A 58c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
58 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4A 62c:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgf4tm1.3Mrt/Fgf4tm1.3Mrt Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
62 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4A 62c Fgfr1-/-:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgfr1tm1Jrt/Fgfr1tm1Jrt Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
62 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4A 38c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * 129X1/SvJ * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgfr2tm1Dor/Fgfr2tm1Dor Spry4tm1Hadj/Spry4+ Tg(CAG-cre)13Miya/0
|
Age:
|
embryonic day 3.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. 38 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4B 74c:
embryonic day 3.75; Spry4tm1Hadj/Spry4 +
(more )
Note: 74 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
4B 74c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.75
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
74 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4B 68c:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgf4tm1.3Mrt/Fgf4tm1.3Mrt Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.75
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
68 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4B 83c:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgfr1tm1Jrt/Fgfr1tm1Jrt Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 3.75
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
83 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4B 77c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * 129X1/SvJ * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgfr2tm1Dor/Fgfr2tm1Dor Spry4tm1Hadj/Spry4+ Tg(CAG-cre)13Miya/0
|
Age:
|
embryonic day 3.75
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. 77 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4C 115c:
embryonic day 4.0; Spry4tm1Hadj/Spry4 +
(more )
Note: 115 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
Specimen
4C 115c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 4.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
115 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4C 111c:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgf4tm1.3Mrt/Fgf4tm1.3Mrt Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 4.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
111 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4C 98c:
(close )
Genetic Background:
|
involves: 129 * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgfr1tm1Jrt/Fgfr1tm1Jrt Spry4tm1Hadj/Spry4+
|
Age:
|
embryonic day 4.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
98 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
Specimen
4C 104c:
(close )
Genetic Background:
|
involves: 129S4/SvJae * 129X1/SvJ * C57BL/6 * CD-1 * MF1
|
Mutant Allele(s):
|
Fgfr2tm1Dor/Fgfr2tm1Dor Spry4tm1Hadj/Spry4+ Tg(CAG-cre)13Miya/0
|
Age:
|
embryonic day 4.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Conditional mutant. 104 cells. Triple labeled: green - Spry4; red - Nanog (assay MGI:6286387); blue - Gata6 (assay MGI:6286386).
|
|