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Gene Expression Data
Assay Details
Assay
Reference: J:175544 Dupuis LE, et al., Altered versican cleavage in ADAMTS5 deficient mice; A novel etiology of myxomatous valve disease. Dev Biol. 2011 Sep 1;357(1):152-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6279800
Gene symbol: Vcan
Gene name: versican
Antibody: anti-DPEAAE
Results
Specimen 1J: embryonic day 15.5 (more )
Note: Triple labeled: green - Vcan (cleaved Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - Acta2 (assay MGI:6279798).
Structure Level Pattern Image Note
TS23: cardiac valve leaflet Present 1J Expression in developing valve cusps
TS23: heart ventricle Present Regionally restricted 1J Expression subjacent to the ventricular endocardium.
TS23: pulmonary artery Present Regionally restricted 1J Expression in the pulmonary artery wall.
Specimen 6C: embryonic day 12.5 (more )
Note: Double labeled: green - Vcan (cleaved Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS20: heart valve primordium Present Regionally restricted 6C Expression in valve cusps.
Specimen 6D: embryonic day 12.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Vcan (cleaved Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS20: heart valve primordium Absent 6D No expression valve cusps.
Specimen 6G: embryonic day 14.5 (more )
Note: Triple labeled: green - Vcan (cleaved Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS22: pulmonary valve Present 6G
Specimen 6H: embryonic day 14.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Triple labeled: green - Vcan (cleaved Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS22: pulmonary valve Weak 6H Authors report significant decrease in cleaved versican compared to wild type littermates.
Specimen 6K: embryonic day 17.5 (more )
Note: Double labeled: green - Vcan (cleaved Vcan); red - Acta2 (smooth muscle actin; assay MGI:6279798).
Structure Level Pattern Image Note
TS25: pulmonary valve Present 6K
Specimen 6L: embryonic day 17.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Vcan (cleaved Vcan); red - Acta2 (smooth muscle actin; assay MGI:6279798).
Structure Level Pattern Image Note
TS25: pulmonary valve Weak 6L Authors report significant decrease in cleaved versican compared to wild type littermates.
Specimen 6U: postnatal adult (more )
Note: Double labeled: green - Vcan (cleaved Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS28: pulmonary valve Present 6U
Specimen 6V: postnatal adult; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Vcan (cleaved Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS28: pulmonary valve Weak 6V Authors report reduced expression of cleaved form in myxomatous valves of mutant mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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