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Gene Expression Data
Assay Details
Assay
Reference: J:175544 Dupuis LE, et al., Altered versican cleavage in ADAMTS5 deficient mice; A novel etiology of myxomatous valve disease. Dev Biol. 2011 Sep 1;357(1):152-64
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6279799
Gene symbol: Vcan
Gene name: versican
Results
Specimen 6A: embryonic day 12.5 (more )
Note: Triple labeled: green - Vcan (intact Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS20: heart valve primordium Present Regionally restricted 6A Prominent expression in endocardial cushions of pulmonary valve cusps. No expression in compacted subendocardial mesenchymal cells.
Specimen 6B: embryonic day 12.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Triple labeled: green - Vcan (intact Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS20: heart valve primordium Strong Regionally restricted 6B Authors report increased expression in subendocardial mesenchymal cells in which there is a loss of compaction.
Specimen 6E: embryonic day 14.5 (more )
Note: Double labeled: green - Vcan (intact Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS22: pulmonary valve Present 6E
Specimen 6F: embryonic day 14.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Vcan (intact Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS22: pulmonary valve Strong 6F Authors report significant increase in intact versican compared to wild type littermates.
Specimen 6I: embryonic day 17.5 (more )
Note: Double labeled: green - Vcan (intact Vcan); blue - Acta2 (smooth muscle actin; assay MGI:6279798).
Structure Level Pattern Image Note
TS25: pulmonary valve Present 6I
Specimen 6J: embryonic day 17.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Vcan (intact Vcan); blue - Acta2 (smooth muscle actin; assay MGI:6279798).
Structure Level Pattern Image Note
TS25: pulmonary valve Strong 6J Authors report significant increase in intact versican compared to wild type littermates.
Specimen 6Q: postnatal day 1 (more )
Note: Double labeled: green - Vcan (intact Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS27: pulmonary valve Present 6Q
Specimen 6R: postnatal day 1; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Vcan (intact Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS27: pulmonary valve Strong 6R Authors report versican accumulation on postnatal day 1.
Specimen 6S: postnatal adult (more )
Note: Double labeled: green - Vcan (intact Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS28: pulmonary valve Present 6S
Specimen 6T: postnatal adult; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Double labeled: green - Vcan (intact Vcan); red - propidium iodide.
Structure Level Pattern Image Note
TS28: pulmonary valve Strong 6T Authors report increased expression in myxomatous valves of mutant mice.
Specimen 9A: embryonic day 17.5 (more )
Note: Triple labeled: green - Vcan (intact Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS25: pulmonary valve Present 9A
Specimen 9B: embryonic day 17.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Note: Triple labeled: green - Vcan (intact Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS25: pulmonary valve Present 9B
Specimen S2A: embryonic day 17.5 (more )
Note: Triple labeled: green - Vcan (intact Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS25: pulmonary valve Present S2A
Specimen S2B: embryonic day 17.5; Adamts5tm1Dgen/Adamts5+ (more )
Note: Triple labeled: green - Vcan (intact Vcan); blue - Acta1 (sarcomeric actin; assay MGI:6279797); red - propidium iodide.
Structure Level Pattern Image Note
TS25: pulmonary valve Present S2B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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